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Informationen zum Autor John Whittall CoEBio3, Manchester Interdisciplinary Biocentre, Manchester University, UK Peter W Sutton GlaxoSmithKline Research and Development Limited, UK Klappentext Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities.Practical Methods for Biocatalysis and Biotransformations is a "how-to" guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. Contents include:* Introduction to the application of biocatalysts in the pharma industry* introduction to molecular biological techniques for biocatalyst development* Lipase and protease methods including enzyme supports and dynamic kinetic resolutions* Nitrile hydrolases and hydratases* Alcohol dehydrogenases and co-factor recycling* Epoxide hydrolases* Amino acid synthesis* Whole cell processes* Other biocatalysts, including monoamineoxidases, aminotransterases, and lyasesPractical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia. Zusammenfassung Practical Methods for Biocatalysis and Biotransformations is a "how-to" guide focusing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. Inhaltsverzeichnis Preface. Abbreviations. List of Contributors. 1 Biotransformations in Small-molecule Pharmaceutical Development (Joseph P. Adams, Andrew J. Collis, Richard K. Henderson and Peter W. Sutton). 2 Biocatalyst Identification and Scale-up: Molecular Biology for Chemists (Kathleen H. McClean). 3 Kinetic Resolutions Using Biotransformations. 3.1 Stereo- and Enantio-selective Hydrolysis of rac-2-Octylsulfate Using Whole Resting Cells of Pseudomonas spp. (Petra Gadler and Kurt Faber). 3.2 Protease-catalyzed Resolutions Using the 3-(3-Pyridine)propionyl Anchor Group: p-Toluenesulfonamide (Christopher K. Savile and Romas J. Kazlauskas). 3.3 Desymmetrization of Prochiral Ketones Using Enzymes (Andrew J. Carnell). 3.4 Enzymatic Resolution of 1-Methyl-tetrahydroisoquinoline using Candida rugosa Lipase (Gary Breen). 4 Dynamic Kinetic Resolution for the Synthesis of Esters, Amides and Acids Using Lipases. 4.1 Dynamic Kinetic Resolution of 1-Phenylethanol by Immobilized Lipase Coupled with In Situ Racemization over Zeolite Beta (Kam Loon Fow, Yongzhong Zhu, Gaik Khuan Chuah and Stephan Jaenicke). 4.2 Synthesis of the (R)-Butyrate Esters of Secondary Alcohols by Dynamic Kinetic Resolution Employing a Bis(tetrafluorosuccinato)-bridged Ru(II) Complex (S.F.G.M. van Nispen, J. van Buijtenen, J.A.J.M. Vekemans, J. Meuldijk and L.A. Hulshof). 4.3 Dynamic Kinetic Resolution 6,7-Dimethoxy-1-methyl-1,2,3,4-tetrahydroisoquinoline (Michael Page, John Blacker and Matthew Stirling). 4.4 Dynamic Kinetic Resolution of Primary Amines with a Recyclable Palladium Nanocatalyst (Pd/AlO(OH)) for Racemization (Soo-Byung Ko, Mahn-Joo Kim and Jaiwook Park). 4.5 Dynamic Kinetic Resolution of Amines Involving Biocatalysis and In Situ Free-radical-mediated Racemization (Stéphane Gastaldi, Ge¿rard Gil and Miche`le P. Bertrand). 4.6 Chemoenzymatic Dynamic Kinetic Resolution of (S)-Ibuprofen (A.H. Kamaruddin and F. Hamzah). 4.7 Dynamic Kinetic...
