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This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Autoimmunity: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Table des matières
Identification and characterization of human autoreactive T cells for autoimmune research.- T cell antigen discovery using cell-based epitope libraries.- Purification, Phenotypic and Functional analyses of Regulatory T Cells in Autoimmunity.- Culturing Murine Bone Marrow-Derived Macrophages.- Macrophage cell culture and characterization by serum human AB in health and disease.- Isolation and culture of rheumatoid arthritis synoviocytes.- Comprehensive Assessment of Neutrophil Function in Autoimmune Diseases.- Neutrophil transmigration analyses for multiplexed drug screening and in-vivo like application testing using an organ on a chip model.- Induction, isolation and characterization of human neutrophil extracellular traps (NETs) to be used in functional assays.- Analysis of cellular metabolism using flow cytometry.- Quantifying mitochondrial metabolism and metabolic fluxes in soft agar cultures.- Assessing mitochondrial respiration of in vitro models for the study of immune-related diseases using a Seahorse analyzer.- Investigating dysregulated X chromosome inactivation in human B cells and plasmacytoid dendritic cells by RNA FISH .- Single-Cell Spatial Transcriptomic of Sjögren s Disease Salivary Glands.- Establishment of Adult Stem Cell-Derived Organoid Cultures: General Principles from the Mouse Gut.- In vitro lymphoid differentiation of human hematopoietic progenitor cells from co-cultures in hypoxia.- Analysis of microRNAs Expression across Cells, Tissues and Body Fluids.- Polysome profiling: a method for global and targeted analysis of mRNA translation.- A Versatile Ribo-seq Workflow for Enhanced Analysis of Ribosome Dynamics.
Résumé
This volume brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Autoimmunity: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
