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This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. Furthermore, this new edition introduces new approaches for quantifying immune-active proteins and peptides and explores the increasing role of multi-omics strategies to help researchers further understand immune function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Immunoproteomics: Methods and Protocols, Third Edition will help advance immune system research and support the development of new diagnostic and therapeutic strategies.
Table des matières
Introduction to the Immune System.- Immunoproteomics: The Evolution from Classical Methods to Emerging Techniques.- Serological Proteome Analysis for Antigen Identification.- Targeted Analyses of MHC-I Peptides using TOMAHAQ.- High-Coverage Immunopeptidomics Using timsTOF Mass Spectrometers with Thunder-DDA-PASEF Boosted by MS2Rescore.- Identification of Antibody-Cognate Antigens via a High-Throughput, Affinity-Chromatography-Based, Shotgun Immunoproteomics Pipeline.- Identification of Disease Characteristic Immune Complex Antigens.- Enrichment of Phosphorylated and Glycosylated MHC Peptides for Mass Spectrometry-Based Neoantigen Analysis.- Characterization of Human Leukocyte Antigen (HLA) Proteins with Integrated Immunopeptidomics and Proteomics Approach.- Artemis: Mass Spectrometry-Based Identification of MHC Presented Peptides Across Alleles, Classes, and Species using Soluble Single-Chain MHC Constructs.- A Novel Machine Learning Post-Processing Filter for Mass Spectrometry-Based Proteogenomics Leveraging Retention Time Deviation and Peptide Physiochemical Properties.- Utilizing Protein Bioinformatics to Delve Deeper into Immunopeptidomic Datasets with AIMS: An Automated Immune Molecule Separator.- Ribosome Profiling for Novel Antigen Discovery.- Multiplexed Detection of Autoantibodies to Glycopeptides Using Microarray.- Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis.- MHCII MAPPS for High-Confident Immunogenicity Risk Assessment of Biotherapeutics.- FLEXMAP 3D Immunoproteomics: A Protocol to Characterize General Human Antibody Profiles Against Pathogens.- Profiling Norovirus Antibody Specificity and Function Using Luciferase Immunoprecipitation System (LIPS) Assays.- Nucleic Acid Programmable Protein Arrays for Autoantibodies Discovery, A Step-by-Step Guide.- Epitope Screening by Hydrogen-Deuterium Exchange Mass
Résumé
This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. Furthermore, this new edition introduces new approaches for quantifying immune-active proteins and peptides and explores the increasing role of multi-omics strategies to help researchers further understand immune function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Immunoproteomics: Methods and Protocols, Third Edition will help advance immune system research and support the development of new diagnostic and therapeutic strategies.
