Correlative Light and Electron MIcroscopy

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

Table des matières

1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
2. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
4. Correlative Light and Electron Microscopy in Parasite Research
5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
7. Correlative Light and Electron Microscopy of GFP
8. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
9. Correlated Light Microscopy and Electron Microscopy (CLEM): Search. and Find!
10. Capturing Endocytic Segregation Events with HPF-CLEM
11. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
14. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
16. Atmospheric Scanning Electron Microscope for Correlative Microscopy
17. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
18. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms

Détails du produit

• Collaboration: Thomas Muller-Reichert (Editeur), Paul Verkade (Editeur)
• Edition: Academic Press
• Langues: Anglais
• Format d'édition: Livre Relié
• Sortie: 17.10.2014
• EAN: 9780124160262
• ISBN: 978-0-12-416026-2
• Dimensions: 195 mm x 25 mm x 240 mm
• Poids: 1311 g
• Thème: Methods in Cell Biology
• Catégories: Sciences naturelles, médecine, informatique, technique > Sciences naturelles en général; Microscopy, SCIENCE / Microscopes & Microscopy