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This new edition brings together some of the latest developments and protocols reflecting the rapidity with which bioreactor technologies are advancing and being applied. Given that the use of bioreactors in cell biology is becoming more commonplace as attempts are made to scale-up production of various types of cells for regenerative medicine and pharmaceutical purposes, this volume provides practical guidance for navigating research projects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioreactors in Stem Cell Biology: Methods and Protocols, Second Edition will benefit both established investigators and newcomers to this dynamic area of study.
Table des matières
- Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines. - Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor. - Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing. - Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors. - High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors. - Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture. - Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor. - Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor. - Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System. - In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor. - Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor. - Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor. - Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts. - Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask. - Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors. - Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model. - A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3). - Applying Stirred Perfusion to 3D Tissue Equivalents to Mimic the Dynamic In Vivo Microenvironment. - Bioreactor-Based Adherent CellsHarvesting from Microcarriers with 3D Printed Inertial Microfluidics.
