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This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
Table des matières
Cell Cycle Progression and Synchronization: An Overview.- The Trypanosomatids Cell Cycle: A Brief Report.- Cell Cycle-Related Clinical Applications.- Flow Cytometry and Cell Cycle Analysis: An Overview.- Synchronization of Cultured Cells to G1, S, G2, and M Phases by Double Thymidine Block.- Cell Synchronization Techniques for Studying Mitosis.- Synchronization of HeLa Cells to Various Interphases Including G1, S, and G2 Phases.- Synchronization of HeLa Cells to Mitotic Subphases.- Cell Cycle Synchronization of Primary and Cultured Articular Chondrocytes.- Synchronization of Leishmania amazonensis Cell Cycle using Hydroxyurea.- Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation.- Synchronization of Saccharomyces cerevisiae Cells for Analysis of Progression through the Cell Cycle.- Cell Cycle Synchrony Methods for Fission Yeast, Schizosaccharomyces pombe.- Analysis of Cell Cycle by Flow Cytometry.- Analysisof Cell Proliferation by Three-Dimensional Culture.- BrdU Incorporation Assay to Analyze the Entry into S Phase.- Assessment of Growth Plate Chondrocytes Proliferative Activity in Embryonic Endochondral Ossification via Ki67 Immunofluorescence.- Detecting Cell Cycle Stage and Progression in Fission Yeast, Schizosaccharomyces pombe.
