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This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Trichoderma reesei: Methods and Protocols serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.
Table des matières
Ecological Genomics and Evolution of Trichoderma reesei.- Industrial Relevance of Trichoderma reesei as an Enzyme Producer.- The Potential of Synthetic Biology for Trichoderma reesei.- Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei.- Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei.- Open the Pores: Electroporation for the Transformation of Trichoderma reesei.- Sexual Crossing of Trichoderma reesei.- CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei.- The Copper-Controlled RNA Interference System in Trichoderma reesei.- Batch Cultivation of Trichoderma reesei.- Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations.- Measurement of Cellulase and Xylanase Activities in Trichoderma reesei.- Flow Cytometry for Filamentous Fungi.- Molecular Identification of Trichoderma reesei.-In Vivo Footprinting Analysis in Trichoderma reesei.- RNA Characterization in Trichoderma reesei.- Proteomic Profiling of the Secretome of Trichoderma reesei.- Transcriptomics in Trichoderma reesei.- The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances.- In Silico Gene Analysis and Oligonucleotide Design for Construction of Expression Vectors.- PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a.- TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations.
