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This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field.
Table des matières
Introduction to the Immune System.- Immunoproteomics Methods and Techniques.- Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification.- Profiling of Cytokine and Chemokine Responses using Multiplex Bead Array Technology.- Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis.- Enriching for Low Abundance Serum Proteins using ProteoMiner(TM) and Protein Level HPLC.- Identification of the Antigen Content of Electroimmunoprecipitates.- Detection of Human Norovirus-Specific Antibodies using the Luciferase Immunoprecipitation System (LIPS) Assay.- Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum.- Antigen Identification for Cell-Binding Antibodies using Ligand Directed Crosslinking and Biotin Transfer.- Whole Genome-Phage Display Libraries: A Powerful Tool for Antigen Discovery.- Multiplexed Detection of Autoantibodies to Glycopeptides using Microarray.- Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification.- Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification.- Enhanced Mass Spectrometry Detection of MHC Peptides.- Enrichment of Phosphorylated MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium Dioxide Particles.- Mass Spectrometric Identification and Molecular Modeling of Glycopeptides Presented by MHC Class I and II Processing Pathways.- Genome Based Bioinformatic Prediction of Major Histocompatibility (MHC).- Enhancing Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted Database Search Approach.- Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics.- Genomics Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases.- Identification of Antigens in Immune Complexes.- Analysis and Characterization of Immune Cells and their Activation Status by Whole-Cell MALDI TOF Mass Spectrometry.- A Molecular Immunoproteomics Approach to Assess the Viral Antigenicity of Influenza.- Immunoproteomic Biomarkers: From Publication to Personalized Medicine.
