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This volume presents techniques used by researchers from all branches of biology to study daily changes at a molecular level in many physiological systems. The chapters are organized into three parts and cover topics such as measuring and modeling physiological and behavioral rhythms; genome-wide analyses in circadian biology, and imaging and manipulating brain clocks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Circadian Clocks: Methods and Protocols is a valuable tool for any researcher interested in learning more about this developing field.
Table des matières
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock.- Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian Biology.- The Assessment of Circadian Rhythms within the Immune System.- Measuring Circadian Rhythms in Human Cells.- Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in Rodents: Forced Swim and Tail Suspension Tests.- Asking the Clock: How to Use Information from Questionnaires for Circadian Phenotyping.- Simple Kinetic Models in Molecular Chronobiology.- Searching Novel Clock Genes using RNAi-Based Screening.- Chromatin Immunoprecipitation (ChIP) from Mouse Liver Nuclei.- Using GRO-seq to Measure Circadian Transcription and Discover Circadian Enhancers.- Circadian Metabolomics from Breath.- A Lipidomics View of Circadian Biology.- Circadian Lipidomics: Analysis of Lipid Metabolites Around-The-Clock.- Phosphoproteome and Proteome Sample Preparation from Mouse Tissues for Circadian Analysis.- Circadian Phosphorylation of CLOCK and BMAL1.- Fluorescence Live Imaging of Drosophila Circadian Pacemaker Neurons.- Monitoring Electrical Activity in Drosophila Circadian Output Neurons.- Light Input to the Mammalian Circadian Clock.- Acute In Vivo Multi-Electrode Recordings from the Mouse Suprachiasmatic Nucleus.- Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices.- Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks.- Computational Analysis of PER2::LUC Imaging Data.- Electrophysiological Approaches to Studying the Suprachiasmatic Nucleus.- Optogenetic Methods for the Study of Circadian Rhythms.
