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This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a var
iety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.
Table des matières
Some Thoughts on Experimental Design.- Comparative Genomics as a Foundation for Evo-Devo Studies in Birds.- A Step-by-Step Guide to Assemble a Reptilian Genome.- Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes.- Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses using the Chicken Skin Paradigm.- Application of a CAGE Method to an Avian Development Study.- CRISPR/Cas9 in the Chicken Embryo.- Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development.- Lentiviral-Mediated Transgenesis in Songbirds.- In Ovo Electroporation Methods in Chick Embryos.- Genetic Manipulation of the Avian Urogenital System using In Ovo Electroporation.- Enhancer Analyses using Chicken Embryo Electroporation.- Transgene Introduction into the Chick Limb Bud by Electroporation.- Chicken Induced Pluripotent Stem Cells: Establishment
and Characterization.- Isolation and Characterization of Chicken Primordial Germ Cells and their Application in Transgenesis.- Handling of Gametes for In Vitro Insemination in Birds.- In Vitro and Ex Ovo Culture of Reptilian and Avian Neural Progenitor Cells.- Lifting the Veil on Reptile Embryology: The Veiled Chameleon (Chamaeleo calyptratus) as a Model System to Study Reptilian Development.- Model Clades versus Model Species: Anolis Lizards as an Integrative Model of Anatomical Evolution.- The Feather Model for Chemo- and Radiation Therapy-Induced Tissue Damage.- An Early Chick Embryo Culture Device for Extended Continuous Observation.- A Sensitive and Versatile In Situ Hybridization Protocol for Gene Expression Analysis in Developing Amniote Brains.- Somitogenesis and Axial Development in Reptiles.- Micro-CT Imaging on Living Alligator Teeth Reveals Natural Tooth Cycling.
Résumé
This volume discusses recent advances in avian and reptilian biology that have caused this diverse field to re-emerge. The chapters in this book are divided into 4 parts: genomics and transcriptomics, genetic manipulation, stem cells, and new model systems. Part I details how to perform genomic and transcriptomic analyses in birds and reptiles; Part II highlights technological advancements in avian genetic manipulation; Part III focuses on methods to handle pluripotent cells; and part IV looks at the emerging models in avian and reptilian developmental biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Avian and Reptilian Developmental Biology: Methods and Protocols explores a variety of approaches and different sauropsid models that will help facilitate communication and collaboration among researchers, which in turn will progress this field forward.
