Biomolecular and Bioanalytical Techniques - Theory, Methodology and Applications

Informationen zum Autor Editor: Dr Vasudevan Ramesh , retired from the Faculty of the School of Chemistry at the University of Manchester, Manchester, UK, after 18 years of distinguished service. He taught Physical Chemistry at all degree levels and pursued an active research programme in Biomolecular NMR spectroscopy. He was a Leverhulme Research Fellow at the University of Leicester and a Visiting Lecturer and Annual Award winner at the Peking University, Beijing, China. Klappentext An essential guide to biomolecular and bioanalytical techniques and their applicationsBiomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique's background, theory, principles, technique, methodology, protocol and applications.The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories.* A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods* Provides the necessary background and skills needed to advance the research methods* Features a structured approach within each chapter* Demonstrates an interdisciplinary approach that serves to develop independent thinkingWritten for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field. Zusammenfassung An essential guide to biomolecular and bioanalytical techniques and their applicationsBiomolecular and Bioanalytical Techniques offers an introduction to! and a basic understanding of! a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research! the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique's background! theory! principles! technique! methodology! protocol and applications.The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules! including small molecules as well as biological macromolecules such as enzymes! antibodies! proteins! peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories.* A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods* Provides the necessary background and skills needed to advance the research methods* Features a structured approach within each chapter* Demonstrates an interdisciplinary approach that serves to develop independent thinkingWritten for students in chemistry! biological! medical! pharmaceutical! forensic and biophysical sciences! Bi...

Table des matières

List of Contributors xix
 
Preface xxiii
 
1 Principles of Health and Safety and Good Laboratory Practice 1
Elaine Armstrong
 
1.1 Introduction 1
 
1.2 Good Laboratory Practice 1
 
1.3 Risk Assessment 2
 
1.4 Chemical Risk Assessment 5
 
1.5 Biological Materials and Genetically Modified Organisms 7
 
1.6 Vacuum Apparatus, Pressure Systems and Associated Glassware 9
 
1.7 Cryogenic Liquefied Gases 9
 
1.8 Compressed Gas Cylinders 10
 
1.9 Electromagnetic Radiation 10
 
1.10 Lasers 11
 
1.11 High Magnetic Fields 11
 
1.12 Sharps 12
 
1.13 Ergonomic Issues 12
 
References 15
 
2 Applications of Chemoinformatics in Drug Discovery 17
Valerie J. Gillet
 
2.1 Significance and Background 17
 
2.2 Computer Representation of Chemical Structures 17
 
2.3 Database Searching 18
 
2.4 Practical Issues on Representation 22
 
2.5 Virtual Screening 22
 
2.6 Ligand-Based Virtual Screening 23
 
2.7 Protein-Ligand Docking 30
 
2.8 Evaluating Virtual Screening Methods 32
 
2.9 Case Studies of Virtual Screening 33
 
2.10 Conclusions 34
 
References 34
 
Further Reading 36
 
3 Bioinformatics and Its Applications in Genomics 37
David J. Parry-Smith
 
3.1 Significance and Short Background 37
 
3.2 Theory/Principles 40
 
3.3 Databases 43
 
3.4 Techniques 45
 
3.5 Applications 53
 
3.6 Concluding Remarks 56
 
References 56
 
Further Reading 57
 
Websites 57
 
4 Gene Cloning for the Analysis of Gene Expression 59
Huw B. Thomas and Raymond T. O'Keefe
 
4.1 Identifying Target Sequence 59
 
4.2 In Silico Design 59
 
4.3 Primer Design 60
 
4.4 Template Preparation 62
 
4.5 Cloning Methods 64
 
4.6 Uses for Cloned DNA Sequences 66
 
4.7 Verifying Cloned Sequences 67
 
4.8 Applications of Gene Constructs 68
 
4.9 Case Study: Cloning of a Human Missense Variant Exon into a Minigene Splicing Vector 69
 
4.10 Case Study: Epitope Tagging of a Yeast Gene 74
 
References 79
 
Further Reading 79
 
Websites 80
 
5 Proteomic Techniques and Their Applications 81
Hsueh-Fen Juan
 
5.1 Significance and Background 81
 
5.2 Principles of Major Proteomics Techniques 82
 
5.3 Methods for Proteomics 85
 
5.4 Applications 92
 
5.5 Concluding Remarks 95
 
Acknowledgements 95
 
References 95
 
Further Reading 99
 
Website Resources 99
 
6 Overproduction, Separation and Purification of Affinity-Tagged Proteins from Escherichia coli 101
Finbarr Hayes and Daniela Barillà
 
6.1 Introduction 101
 
6.2 Selecting an Affinity Tag: Glutathione-S-Transferase, Maltose-Binding Protein and Hexa-Histidine Motifs 103
 
6.3 The pET Vector Series: Archetypal Expression Vectors in E. coli 105
 
6.4 IMAC of a His6-Tagged Protein: Example Methodology with the ParF DNA Segregation Protein 107
 
6.5 Production and Purification of a GST-Tagged Protein: Example Methodology with the C-Terminal Domain of Yeast RNA Polymerase II 112
 
6.6 Further Purification of Tagged Proteins 114
 
6.7 Alternative Hosts for Protein Production 116
 
6.8 Concluding Remarks 116
 
Acknowledgements 117
 
References 117
 
Further Reading 121
 
7 Chromatography: Separation Techniques in Biology 123
W John Lough and Mark Carlile
 
7.1 Introduction to Chromatographic Separation 1

Détails du produit

• Auteurs: V Ramesh, Vasudevan Ramesh
• Collaboration: Vasudeva Ramesh (Editeur), Vasudevan Ramesh (Editeur), Ramesh Vasudevan (Editeur)
• Edition: Wiley, John and Sons Ltd
• Langues: Anglais
• Format d'édition: Livre Relié
• Sortie: 30.04.2019
• EAN: 9781119483960
• ISBN: 978-1-119-48396-0
• Pages: 576
• Thème: *Unallocated*
• Catégories: Sciences naturelles, médecine, informatique, technique > Chimie > Chimie théorique; Chemie, Analytische Chemie, Life Sciences, Biowissenschaften, chemistry, Analytical Chemistry, Zell- u. Molekularbiologie, Cell & Molecular Biology, Bioanalytik, Pharmaceutical & Medicinal Chemistry, Pharmazeutische u. Medizinische Chemie, Biomolekül