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This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a widerange of assay types all on the cutting edge of diagnostic bacteriology.
Table des matières
Whole Genome Enrichment using RNA Probes and Sequencing of Chlamydia Trachomatis Directly from Clinical Samples.- Characterization of Sinus Microbiota by 16S Sequencing from Swabs.- Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).- Detection of Helicobacter pylori DNA in Formalin-fixed Paraffin Embedded Gastric Biopsies using Laser Micro-dissection and qPCR.- Mycobacterial Load Assay.- Defining Diagnostic Biomarkers using Shotgun Proteomics and MALDI-TOF Mass Spectrometry.- Detection and Typing of 'Candidatus Phytoplasma' spp. in Host DNA Extracts Using Oligonucleotide-Coupled Fluorescent Microspheres.- Detection of Helicobacter Pylori in the Gastric Mucosa by Fluorescence in vivo Hybridization.- Rapid Antibiotic Susceptibility Testing for Urinary Tract Infections.- Detection and Differentiation of Lyme Spirochetes and other Tick-borne Pathogens from Blood using real-time PCR with Molecular Beacons.- Methods for Real-time PCR-based Diagnosis of Chlamydia Pneumoniae, Chlamydia Psittaci and Chlamydia Abortus Infections in an Opened Molecular Diagnostic Platform.- Real-time PCR to Identify Staphylococci and Assay for Virulence from Blood.- Multiplex Peptide Nucleic Acid Fluorescence in situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis.- A closed-tube Loop-Mediated Isothermal Amplification Assay for the Visual Endpoint Detection of Brucella spp. and Mycobacterium Avium Subsp. Paratuberculosis.- Highly Specific Ligation-Dependent Microarray Detection of Single Nucleotide Polymorphisms.- Multilocus Sequence Typing (MLST) for Cronobacter spp..- Diagnostic Bacteriology: Raman Spectroscopy.
Résumé
This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike.
