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This volume provides an up-to-date compilationof current methodological approaches utilized for the exploration of nucleolarstructure and function. Chapters covera diversity of protocols that include imaging of the nucleolus, analysis ofribosomal RNA transcription and processing, and genomics and proteomics of thenucleolus. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practicalhandbook to facilitate the investigation of this nuclear compartment at theadvanced level.
Table des matières
The Relationship between Human NucleolarOrganizer Regions and Nucleoli, Probed by 3D-Immunofish.- Correlative Light andElectron Microscopy of Nucleolar Transcription in Saccharomyces Cerevisiae.- High-Throughput Live-Cell MicroscopyAnalysis of Chromosome Domain - Nucleolus Associations in S. Cerevisiae.- Quantitative Immunofluorescence Analysis ofNucleolus-Associated Chromatin.- Visualization of The Nucleolus in Living Cellswith Cell-Penetrating Fluorescent Peptides.- Purification of S. Cerevisiae RNA Polymerase I forStructural and Functional Studies.- Analysis of S. Cerevisiae RNA Polymerase I Transcription In Vitro.- Preparation of Chromatin Templates to Study RNA PolymeraseI Transcription In Vitro.- Measurementof Rrna Synthesis and Processing by 4su-Tagging.- Metabolic Labeling in the Studyof Mammalian Ribosomal RNA Synthesis.- Quantitative Northern Blot Analysis ofMammalian Rrna Processing.- CompleteSequence Construction of the Highly Repetitive Ribosomal RNA Gene Repeats inEukaryotes using Whole Genome Sequence Data.- Analysis of Rdna Methylation in Arabidopsis By 2D Gel Electrophoresis.- Fluorescence-ActivatedSorting of Arabidopsis Nucleoli.- Purificationof RNA Polymerase I-Associated Chromatin from Yeast Cells.- Bioinformatic Analysisof Chip-Seq Data on the Repetitive Rrna Gene.- Deep Sequencing Analysis of NucleolarSmall Rnas: Isolation and Library Preparation.- Deep Sequencing Analysis ofNucleolar Small RNAs: Bioinformatics.- Quantitative Proteomic Analysis of theHuman Nucleolus.- Assessing Nucleolar Localization of Proteins: BioinformaticAnalysis of Proteomics Data.
Résumé
This volume provides an up-to-date compilationof current methodological approaches utilized for the exploration of nucleolarstructure and function. Chapters covera diversity of protocols that include imaging of the nucleolus, analysis ofribosomal RNA transcription and processing, and genomics and proteomics of thenucleolus.
