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In the last few years, technologies that allow for monitoring and manipulating neuronal networks at a single cell resolution have been rapidly expanding. In Neuronal Network Analysis: Concepts and Experimental Approaches, experts in the field provide an overview of some of the most advanced techniques for the study of neuronal circuits, highlighting current technical limitations and providing a vision of what is yet to come. Broken into six convenient, interdependent section, this volume covers optical approaches, electrophysiological approaches, both in vitro and in vivo, optogenetics, morpho-functional approaches, and neuronal network analysis in humans. As a volume in the Neuromethods series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.
Authoritative and cutting-edge, Neuronal Network Analysis: Concepts and Experimental Approaches will be useful for early career neuroscientists, as well as for experienced neuroscientists, by providing a practical framework for studying the brain as an organ while maintaining an appreciation for its cellular diversity.
Table des matières
Optical Interrogation of Neural Circuits.- In Vivo Functional Imaging of the Olfactory Bulb at Single Cell Resolution.- Two-Photon Imaging of Neural Activity in Awake, Head-Fixed Mice.- Spatial Light Modulators for Complex Spatio-Temporal Illumination of Neuronal Networks.- Voltage-Sensitive Dye Imaging of Cortical Function In Vivo.- An Introduction to In Vitro Slice Approaches for the Study of Neuronal Circuitry.- Extracellular Recordings of Synaptic Plasticity and Network Oscillations in Hippocampal Slices.- Use of Dynamic-Clamp as a Tool to Reveal the Computational Properties of Single Neurons Embedded in Cortical Circuits.- Fast Perfusion Methods for the Study of Ligand-Gated Ion Channels.- Electrophysiological Approaches for Studying Neuronal Circuits In Vivo.- Determination of Cortical Circuit Function Using Current Source-Density (CSD) Analysis In Vivo.- Intracellular Whole-Cell Patch-Clamp Recordings of Cortical Neurons in Awake Head-Restrained Mice.- Investigating Sleep Homeostasis with Extracellular Recording of Multiunit Activity from the Neocortex in Freely-Behaving Rats.- Analysis of Hippocampal Memory Replay Using Neural Population Decoding.- High Speed Videography of Embodied Active Sensing in the Rodent Whisker System.- Light-Activated Ion Pumps and Channels for Temporally-Precise Optical Control of Activity in Genetically-Targeted Neurons.- Integrated Optogenetic and Electrophysiological Dissection of Local Cortical Circuits In Vivo.- Combining Optical Stimulation with Extracellular Electrophysiology in Behaving Mice.- Morphological Approaches to the Anatomical Dissection of Neuronal Circuits.- The Isotropic Fractionator: A Fast, Reliable Method to Determine Numbers of Cells in the Brain or Other Tissues.- Morpho-Functional Mapping of Cortical Networks inBrain Slice Preparations Using Paired Electrophysiological Recordings.- Combining Transcranial Magnetic Stimulation with Electroencephalography to Study Human Cortical Excitability and Effective Connectivity.- Biophysically Principled Computational Neural Network Modeling of Magneto-/Electro-Encephalography (MEG/EEG) Measured Human Brain Oscillations.
Résumé
In recent years, technologies used to monitor and manipulate neuronal networks at single-cell resolution have expanded rapidly. This book examines the latest techniques in the field as well as highlighting current limitations and positing future developments.
