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The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.
Table des matières
Preparation of 2 nm Gold Nanoparticles for In vitro and In vivo Applications.- DNA Conjugation to Nanoparticles.- Conjugation of Nanoparticles to Proteins.- Water-Solubilization and Functionalization of Semiconductor Quantum Dots.- Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates.- Reliable Methods for Silica Coating of Au Nanoparticles.- Surface Modifications by Polymers for Biomolecule Conjugation.- Functionalization Protocols of Silicon Micro-Nano-Mechanical Biosensors.- Stability and Aggregation Assays of Nanoparticles in Biological Media.- Electrochemical Measurements of DNA Melting on Surfaces.- Formation and Characterization of Nanoparticle-Protein Corona.- Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Absorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution.- Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment.- Immunochemistry, Electron Tomography and Energy Dispersive X-Ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles.- Zwitterion Siloxane to Passivate Silica Against Non-Specific Protein Adsorption.- Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles.- Polyaspartic Acid Coated Iron Oxide Nanoprobes for PET/MRI Imaging.- Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy.- Non-Covalent Intracellular Drug Delivery of Hydrophobic Drugs on Au NPs.- Modification of Carbon Nanotubes for Gene Delivery Vectors.- Lipid-Based Nanoparticles as Non-Viral Gene Delivery Vectors.- Stabilizing Gold Nanoparticle Bioconjugates in Physiological Conditions by PEGylation.
Résumé
The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.
