Basic Cell Culture Protocols

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Table des matières

Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line.- Detection of Mycoplasma Contaminations.- Eradication of Mycoplasma Contaminations.- Authentication of Scientific Human Cell Lines.- Cytogenetic Analysis of Cell Lines.- Human and Mouse Hematopoietic Colony-Forming Cell Assays.- Isolation and Culture of Murine Macrophages.- Isolation and Culture of Human Macrophages.- Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture.- In Vitro Generation of Lymphocytes From Embryonic Stem Cells.- Hematopoietic Development of Human Embryonic Stem Cells in Culture.- Generation of Murine Stromal Cell Lines.- Culture of Human and Mouse Mesenchymal Cells.- Isolation, Purification, and Cultivation of Murine and Human Keratinocytes.- Isolation and Culture of Primary Human Hepatocytes.- Primary Kidney Proximal Tubule Cells.- Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity.- Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue.- Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells.- Adult Ventricular Cardiomyocytes.- Isolation and Culture of Primary Endothelial Cells.- Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions.- Isolation and Characterization of Side Population Cells.- Scalable Production of Embryonic Stem Cell-Derived Cells.

Résumé

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Texte suppl.

"This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it. The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture."-Doody's Health Sciences Book Review Journal

From Reviews of the Second Edition. . .

"...a highly prized collection that no cell culture laboratory should be without, so much so that it is likely to be 'borrowed' and never returned!"

-Retinoids & Lipid Soluble Vitamins in Clinical Practice

"...any laboratory involved in cell culture will find this book of use..."

-Microbiology Today

"...would be most useful to have in the laboratory itself...and a valuable reference resource in a library." Four Stars

-Doody's Health Sciences Book Review Journal

"...an excellent starting point for those who are interested in learning the basics of cell culture and the types of manipulations that make them useful."

-Journal of Chemical Neuroanatomy

Commentaire

"This book is written by experienced authors who understand the practical needs of bench scientists. All laboratory personnel who perform cell culture on a regular basis will benefit from it. The authors provide summaries, keywords, helpful introductions, lists of necessary materials and equipment, and detailed protocols for culturing a wide-variety of cell types. The chapters on manipulating pluripotent stem cells will be of great value to researchers interested in regenerative medicine and cell engineering. This book is an important and valuable contribution to the field of cell biology and the art of manipulating cells in culture."-Doody's Health Sciences Book Review Journal

From Reviews of the Second Edition. . .

"...a highly prized collection that no cell culture laboratory should be without, so much so that it is likely to be 'borrowed' and never returned!"
-Retinoids & Lipid Soluble Vitamins in Clinical Practice

"...any laboratory involved in cell culture will find this book of use..."
-Microbiology Today

"...would be most useful to have in the laboratory itself...and a valuable reference resource in a library." Four Stars
-Doody's Health Sciences Book Review Journal

"...an excellent starting point for those who are interested in learning the basics of cell culture and the types of manipulations that make them useful."
-Journal of Chemical Neuroanatomy

Détails du produit

• Collaboration: Chery D Helgason (Editeur), Cheryl D Helgason (Editeur), Cheryl D. Helgason (Editeur), L Miller (Editeur), L Miller (Editeur), Cindy L. Miller (Editeur)
• Edition: Springer, Berlin
• Langues: Anglais
• Format d'édition: Livre de poche
• Sortie: 01.01.2014
• EAN: 9781627039307
• ISBN: 978-1-62703-930-7
• Pages: 371
• Dimensions: 144 mm x 20 mm x 228 mm
• Poids: 561 g
• Illustrations: XII, 371 p.
• Thèmes: Methods in Molecular Biology; Methods in Molecular Biology
• Catégories: Sciences naturelles, médecine, informatique, technique > Biologie > Microbiologie; B, Development, Enzyme, Biomedical and Life Sciences, Cell Biology, stem cells, Cell Culture, tissue, Stem Cell, Neural Stem Cells, cell lines, human cell lines