Fr. 64.00

Human skeletal remains:development of DNA extraction and typing assays - DNA extraction and typing of old human bones

Anglais, Allemand · Livre de poche

Expédition généralement dans un délai de 2 à 3 semaines (titre imprimé sur commande)

Description

En savoir plus

Efficient DNA extraction procedures, as well as accurate DNA amplification, are critical steps involved in the process of successful DNA analysis of skeletal samples. Unfortunately, at present there is not an infallible method to recover DNA from very degraded samples due to variations in DNA yield from bone fragments that may be attributed to heterogeneity within a bones. In this study different types of human bones ranging in age from few months to 90 post mortem years, found in various states of preservation and conserved in different places, were analyzed. We developed a modified silica based spin columns protocol, associated to the typing of conventional Short Tandem Repeats (STRs) and smaller Polymerase Chain Reaction (PCR) products (Mini-STRs) redesigned by our laboratory by moving forward and reverse primers in close proximity to the STR repeat region. Our modified protocol was successfully employed to extract DNA from long bones of different ages and preservation state. Importantly the use of phenol chloroform consistently increased the amount of DNA that could be extracted from long bones, preventing that waste material interferes with columns or magnetic beads.

A propos de l'auteur










Giulia Filippini has obtained her Master degree in Sanitary Biology at University of Padua and she got PhD in Forensic Medicine and Science at University of Verona in 2010. She has acquired of experience in Forensic Genetic field, producing more than ten peer- reviewed articles. Giulia is carrying on with her studies as post-doctoral researcher.

Détails du produit

Auteurs Domeni De Leo, Domenico De Leo, Giuli Filippini, Giulia Filippini, Stefani Turrina, Stefania Turrina
Edition LAP Lambert Academic Publishing
 
Langues Anglais, Allemand
Format d'édition Livre de poche
Sortie 05.04.2012
 
EAN 9783848436972
ISBN 978-3-8484-3697-2
Pages 80
Dimensions 150 mm x 220 mm x 4 mm
Poids 123 g
Catégorie Sciences naturelles, médecine, informatique, technique > Biologie > Génétique

Commentaires des clients

Aucune analyse n'a été rédigée sur cet article pour le moment. Sois le premier à donner ton avis et aide les autres utilisateurs à prendre leur décision d'achat.

Écris un commentaire

Super ou nul ? Donne ton propre avis.

Pour les messages à CeDe.ch, veuillez utiliser le formulaire de contact.

Il faut impérativement remplir les champs de saisie marqués d'une *.

En soumettant ce formulaire, tu acceptes notre déclaration de protection des données.