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Zusatztext '...Practical advice is given by experts in the different topics! together with lists of useful literature'Librairie Klappentext This book describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Such techniques form the basis of routine tests in chemical! biological! and medical science laboratories. Practical advice is given! from experts in the different topics! together with lists of useful literature. Zusammenfassung Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Practical advice is given, from experts in the different topics, together with lists of useful literature. Introduction to light absorption; visible and ultraviolet spectra; Fluorescence principles and measurement; Time resolved fluorescence and phosphorescence spectroscopy; An introduction to circular dichroism; Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods; Steady state kinetics; Spectrophotometric assays; Stopped flow spectroscopy; Stopped flow fluorescence; Stopped flow circular dichroism; Spectrophotometry and fluorimetry of cellular compartments and intracellular processes; Use of optical spectroscopic methods to study the thermodynamic stability of proteins; The use of spectroscopic techniques in the study of protein stability
Résumé
Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Practical advice is given, from experts in the different topics, together with lists of useful literature.
