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The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
Table des matières
DisMeta - a Meta Server for Construct Design and Optimization.- Stable Expression Clones and Auto-Induction for Protein Production in E. Coli.- High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli.- Medium Throughput Production of Recombinant Human Proteins: Ligation Independent Cloning.- Medium Throughput Production of Recombinant Human Proteins: Protein Production in E. coli.- Medium Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells.- OmniBac: Universal Multigene Transfer Plasmids for Baculovirus Expression Vector Systems.- Multiprotein Complex Production in Insect Cells by Using Polyproteins.- Expression Screening in Mammalian Suspension Cells.- Cell-free Expression of Protein Complexes for Structural Biology.- Cell-Free Protein Synthesis for Functional and Structural Studies.- Insoluble Protein Purification with Sarkosyl: Facts and Precautions.- Estimation of Crystallization Likelihood Through a Fluorimetric Thermal Stability Assay.- CrystalDirect(TM): A Novel Approach for Automated Crystal Harvesting Based on Photoablation of Thin Films.- Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropy.- Applications of NMR-Based PRE and EPR-Based DEER Spectroscopy to Homodimer Chain Exchange Characterization and Structure Determination.- A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labelled Mutants for NMR Studies of High Molecular Weight Proteins.- High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach.- Measuring Spatial Restraints on Native Protein Complexes using Isotope-Tagged Chemical Cross-Linking and Mass Spectrometry.- Modeling of Proteins and Their Assemblies with the Integrative Modeling Platform.- The Quality and Validation of Structures from Structural Genomics.- Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb.- Mespeus, A Databaseof Metal Interactions with Proteins.- High-Quality Macromolecular Graphics on Mobile Devices: A Quick Starter's Guide.
Résumé
The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applicationsemphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Structural Genomics: General Applicationsaims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.
