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Informationen zum Autor Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements. Klappentext Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures Zusammenfassung A comprehensive source of methods for the analysis cilia and flagella. It covers protocols for cilia and flagella across systems and species. It is suitable for clinicians interested in respiratory disease! male infertility and other syndromes! who need to learn biochemical and genetic approaches to studying cilia! flagella and related structures. Inhaltsverzeichnis MCB, 92 1. Bioinformatic Approaches to Dynein Heavy Chain Classification Toshiki Yagi 2. Identification and Characterization of Dynein Genes in Tetrahymena David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang, Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson, Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva, Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva, Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson, Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai 3. Purification of Axonemal Dyneins and Dynein-Associated Components from Chlamydomonas Stephen M. King 4. Purification of Dyneins from Sperm Flagella Kazuo Inaba and Katsutoshi Mizuno 5. Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression System Takahide Kon, Tomohiro Shima, and Kazuo Sutoh 6. Biophysical Measurements on Axonemal Dyneins Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa 7. Protein Electroporation into Chlamydomonas for Mutant Rescue Masahito Hayashi and Ritsu Kamiya 8. Analysis of the Role of Nucleotides in Axonemal Dynein Function Chikako Shingyoji 9. The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas Flagella by Axonemal Kinases and Phosphatases Candice A. Elam, Winfield S. Sale, and Maureen Wirschell 10. Analysis of Redox-Sensitive Dynein Components Ken-ichi Wakabayashi 11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding Proteins Christen DiPetrillo and Elizabeth Smith 12. Isolation and Analysis of Radial Spoke Proteins Pooja Kelekar, Mei Wei, and Pinfen Yang 13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii David R. Mitchell and Brandon Smith ...
