Arabidopsis Protocols, 2nd Edition

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

Inhaltsverzeichnis

Growing Arabidopsis.- Growth of Plants and Preservation of Seeds.- Prevention and Control of Pests and Diseases.- Growth of Other Species Related to Arabidopsis thaliana.- Grafting.- Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells.- Genetic Analyses.- Genetic Analysis.- QTL Analysis.- EMS Mutagenesis of Arabidopsis.- Low-Resolution Mapping of Untagged Mutations.- Gene Identification and Cloning by Molecular Marker Mapping.- High-Throughput TILLING for Arabidopsis.- Genotyping and Mapping With High-Density Oligonucleotide Arrays.- Forward Genetic Screening of Insertional Mutants.- PCR-Based Screening for Insertional Mutants.- Cytogenetic Analyses of Arabidopsis.- Using Information From Public Arabidopsis Databases to Aid Research.- Transient and Stable Transformation.- In Planta Agrobacterium-Mediated Transformation by Vacuum Infiltration.- Transient Expression Assay by Agroinfiltration of Leaves.- Functional Analysis of Transcription Factors by Microparticle Bombardment.- Transfection Assays With Protoplasts Containing Integrated Reporter Genes.- Chloroplast Genetic Engineering Via Organogenesis or Somatic Embryogenesis.- ?-Glucuronidase as Reporter Gene.- Use of Fluorescent Proteins as Reporters.- Luciferases as Reporter Genes.- Aequorin as a Reporter Gene.- Applications of Chemical-Inducible Expression Systems in Functional Genomics and Biotechnology.- Transcriptomics.- RNA Extraction.- Target Preparation for DNA Microarray Hybridization.- Statistical Issues in Microarray Data Analysis.- Preparation and Quality Assessment of RNA From Cell-Specific Samples Obtained by Laser Microdissection.- Proteomics.- Protein Isolation and Second-Dimension Electrophoretic Separation.- Isolation of Nuclear Proteins.- Purification and Fractionationof Membranes for Proteomic Analyses.- Protein Microsequencing.- Peptide-Based Phosphoproteomics With Immobilized Metal Ion Chromatography.- Metabolomics.- Metabolite Profiling in Arabidopsis.- Hormone Profiling in Arabidopsis.

Zusammenfassung

This collection of readily reproducible Arabidopsis protocols has been updated to reflect recent advances in plant biology, the completion of the Arabidopsis genome sequence-essential for studying plant function, and the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics. The authors have included nearly all techniques developed in Arabidopsis, others recently adapted from the traditional work in crop species, and the most recent ones using Arabidopsis as a model system.

Zusatztext

From the reviews of the first edition:
". . .a comprehensive collection of significant new methods as well as updated standard protocols. . .written by experienced authors and internationally recognized experts."-Journal of Plant Physiology
"...recommend[ed]...provides a set of enduring methods and is comprehensive enough for instant use in the lab" -Trends in Plant Science

"...a 'must have' for every laboratory involved in Arabibopsis" -Quarterly Review of Biology

"...a 'must' for those labs working with not just Arabidopsis specifically but plants in general..." -Microbiology Today

"...a must for anybody moving into the Arabidopsis field and setting up a new lab...extremely useful" -Genetical Research

"...especially valuable for detailed step-by-step procedures including many important notes...an indispensable guide, which should be at reach on the shelf in each laboratory where the plant molecular-genetic methods in Arabidopsis, and not only Arabidopsis, are used."- Biologia Plantarum
"...should prove to be highly helpful laboratory companion for beginners and established researchers in molecular biology, particularly those whose research deal with Arabidopsis and related plants." - Plant Science Bulletin
From the reviews of the second edition:
"Arabidopsis Protocols is another volume in Humana’s series, Methods in Molecular Biology. … It is … important to provide both experienced researchers and those new to the use of Arabidopsis, with single volumes that describe a wide range of experimental protocols. This book does that admirably. It is written by renowned experts in their respective fields … . Most chapters include useful Figures to illustrate the text. So I would heartily recommend this to any lab serious about carrying out work with Arabidopsis … ." (Keith Lindsey, Molecular Biotechnology, Vol. 36, 2007)

Bericht

From the reviews of the first edition:
". . .a comprehensive collection of significant new methods as well as updated standard protocols. . .written by experienced authors and internationally recognized experts."-Journal of Plant Physiology
"...recommend[ed]...provides a set of enduring methods and is comprehensive enough for instant use in the lab" -Trends in Plant Science

"...a 'must have' for every laboratory involved in Arabibopsis" -Quarterly Review of Biology

"...a 'must' for those labs working with not just Arabidopsis specifically but plants in general..." -Microbiology Today

"...a must for anybody moving into the Arabidopsis field and setting up a new lab...extremely useful" -Genetical Research

"...especially valuable for detailed step-by-step procedures including many important notes...an indispensable guide, which should be at reach on the shelf in each laboratory where the plant molecular-genetic methods in Arabidopsis, and not only Arabidopsis, are used."- Biologia Plantarum
"...should prove to be highly helpful laboratory companion for beginners and established researchers in molecular biology, particularly those whose research deal with Arabidopsis and related plants." - Plant Science Bulletin
From the reviews of the second edition:
"Arabidopsis Protocols is another volume in Humana's series, Methods in Molecular Biology. ... It is ... important to provide both experienced researchers and those new to the use of Arabidopsis, with single volumes that describe a wide range of experimental protocols. This book does that admirably. It is written by renowned experts in their respective fields ... . Most chapters include useful Figures to illustrate the text. So I would heartily recommend this to any lab serious about carrying out work with Arabidopsis ... ." (Keith Lindsey, Molecular Biotechnology, Vol. 36, 2007)

Produktdetails

• Mitarbeit: Julio Salinas (Herausgeber), Jose J. Sanchez-Serrano (Herausgeber)
• Verlag: Springer, Berlin
• Sprache: Englisch
• Produktform: Fester Einband
• Erschienen: 26.05.2009
• EAN: 9781588293954
• ISBN: 978-1-58829-395-4
• Seiten: 496
• Gewicht: 1166 g
• Illustration: 496 p. 136 illus., 1 illus. in color.
• Serien: Methods in Molecular Biology; Methods in Molecular Biology
• Themen: Naturwissenschaften, Medizin, Informatik, Technik > Geowissenschaften; Sachbuch > Natur, Technik > Natur und Gesellschaft: Allgemeines, Nachschlagewerke; Botanik und Pflanzenwissenschaften, B, Nature, Transcription, Environment, hybridization, Botany, Genetic Engineering, Biomedical and Life Sciences, Botany & plant sciences, Environmental Sciences, Plant Science, Plant Sciences, Popular Science in Nature and Environment, genes