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In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
Zusammenfassung
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells.
Zusatztext
"This book offers a welcome text which is value to anybody undertaking human cell culture." - Cell Biochemistry and Function
"...a very useful general text for researchers involved in human cell cultures. -Immunology News
From Reviews of the First Edition...
"...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information." -Trends in Cell Biology
"...an invaluable and highly recommended resource for cell culturists setting up new systems." -Molecular Biotechnology
"...will be of significance to a very wide range of scientists." -ISSX Newsletter
"...has a wealth of down-to-earth, ready-to-use information." -Modern Pathology
"..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered." -BTS Newsletter
