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This third edition volume explores the latest advancements in genotoxicity testing and combines established assays with emerging technologies. Organized into five thematic sections, the book examines gene mutation assays, assays for chromosomal abnormalities, tests for primary DNA damage, assays in alternatives to animal models, as well as multipronged approaches to the assessment of genotoxicity. Written for the highly successful Methods in Molecular Biology series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Third Edition serves as a comprehensive resource for toxicologists, regulatory scientists, academicians, and students, dedicated to ensuring chemical safety in an evolving landscape of technological and scientific innovation.
Inhaltsverzeichnis
Bacterial Reverse Mutation Test - Ames Test.- The AMES II and Ames MPFTM Tests: Miniaturized Liquid Microplate Formats of the Ames Test in Petri Dishes.- In Vitro Mouse Lymphoma Cell (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay.- Erythrocyte-Based Pig-a Gene Mutation Assay.- Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes.- In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests.- In Vivo Micronucleus and Chromosomal Aberration Assay in Mouse Bone Marrow.- Chromosomal Aberration Test in Human Lymphocytes.- Micronucleus Assay in Human Lymphocytes and Exfoliated Buccal Cells.- 3D Liver Models for Genotoxicity Testing.- Flow Cytometric Determination of Micronucleus Frequency.- Comparative Genomic Hybridization (CGH) in Genotoxicology: From the Basics to Modern Approaches.- 32P-Postlabeling Analysis of DNA Adducts.- Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers.- The Alkaline Comet Assay for Assessment of DNA Damage.- Application of the Comet Assay in Advanced In Vitro Models.- The Comet Assay in Human Biomonitoring.- The Comet Assay in Nano-Ecogenotoxicology.- Micronucleus Assay with Tetrad Cells of Tradescantia.- Application of Allium cepa Test for Genetic Toxicology.- Methods to Study DNA Damage Using Drosophila melanogaster.- The Wing-Spot and the Comet Tests as Useful Assays Detecting Genotoxicity in Drosophila.- Organoids for Genotoxicity Assessment.- Modern Approaches in Genetic Toxicology Research: Insights from Next-Generation-Based RNA Sequencing.- Methods to Quantify the MicroRNA and Study Their Role in Genotoxic Stress.- In Silico Approaches in Predictive Genetic Toxicology.- Artificial Intelligence and Machine Learning-Based Approaches for Genetic Damage Prediction.
