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Cytotoxicity experiments are performed in order to assess the possible toxic effects and safety profiles of chemical, physical and biological agents. There are several mechanisms by which a substance may lead to cellular toxicity, including impairment of cellular functions, disruption of the cellular membrane and/or cellular signalling pathways or inducing an immune response. Cytotoxicity testing involves both in vitro and in vivo methods. In vitro methods offer a rapid and cost-effective approach, while in vivo methods provide a more comprehensive assessment. Rigorous cytotoxicity testing is essential for meeting regulatory standards, enhancing user safety, and improving the product quality of drugs, cosmetics and medical devices. Ensuring the accuracy and reliability of data obtained from in vitro cytotoxicity studies is critical for evaluating the safety profiles of different agents. In the past years, many in vitro methods have been developed to evaluate the viability of cells, and each method has its advantages, disadvantages, strengths and limitations. There are different methods for determining cytotoxicity. They can be summarized as "Staining Methods", "Colorimetric Methods", "Fluorometric Methods", "Luminometric Methods" and "Other Methods". The researchers should choose the most appropriate method for their research by considering the cell type they will use, the dose and properties of the chemical, physical and biological agents, and the mechanism through which it causes cell death (such as apoptosis, necrosis, autophagy). This book mainly focuses on cytotoxicity determination as the primary and crucial step in evaluating toxicity. In this book, the readers will find traditional and new methods used to evaluate cytotoxicity. We believe that readers will get qualified scientific knowledge and a general overview of the importance of cytotoxicity testing.
