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Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Inhaltsverzeichnis
Noncontact Laser Microdissection and Pressure Catapulting.- Applications of the Universal DNA Microarray in Molecular Medicine.- Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization.- Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays.- Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology.- Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader®.- Controlled Agitation During Hybridization.- Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Conductance Regulator Gene by Luminex® xMAP(TM) Suspension Array.- Protein Microarray-Based Screening of Antibody Specificity.- Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection.- Allergen Microarrays.- Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples.- Multiplexed Cytokine Sandwich Immunoassays Clinical Applications.- Validation and Quality Control of Protein Microarray-Based Analytical Methods.- Tissue Microarrays.
Zusammenfassung
Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Zusatztext
From the reviews:
"a volume for the specialist with a fascination for the optimal collection and analysis of large data sets in microarray format."-SGM Quarterly
"...there is no other comparable book on the market...highly recommended to anyone interested in this rapidly evolving field of technology..." - Doody's Health Science Book Review Journal
"Microarray technology has become a mainstay of biological research. … The book is divided into 15 chapters … . Overall this book offers a good read to both clinicians and researchers at the bench who wish to enter the field of microarrays. The format of the book also provides a good roadmap to graduate students wishing to translate aspects of their research to methods amenable to microarrays." (Sridar V. Chittur, Clinical Chemistry, Vol. 52 (6), June, 2006)
"Microarrays in Clinical Diagnostics … is a collection of fifteen chapters by authors from both industry and academia, surveying current applications of microarray-based technologies in diagnostics … . provides protocols in the successful style of the Methods in Molecular Biology series, with clarity and sufficient technical detail so that both novice and current microarray users are sure to benefit from this book." (Nicolas Winssinger, ChemMedChem, Issue 1, 2006)
Bericht
From the reviews:
"a volume for the specialist with a fascination for the optimal collection and analysis of large data sets in microarray format."-SGM Quarterly
"...there is no other comparable book on the market...highly recommended to anyone interested in this rapidly evolving field of technology..." - Doody's Health Science Book Review Journal
"Microarray technology has become a mainstay of biological research. ... The book is divided into 15 chapters ... . Overall this book offers a good read to both clinicians and researchers at the bench who wish to enter the field of microarrays. The format of the book also provides a good roadmap to graduate students wishing to translate aspects of their research to methods amenable to microarrays." (Sridar V. Chittur, Clinical Chemistry, Vol. 52 (6), June, 2006)
"Microarrays in Clinical Diagnostics ... is a collection of fifteen chapters by authors from both industry and academia, surveying current applications of microarray-based technologies in diagnostics ... . provides protocols in the successful style of the Methods in Molecular Biology series, with clarity and sufficient technical detail so that both novice and current microarray users are sure to benefit from this book." (Nicolas Winssinger, ChemMedChem, Issue 1, 2006)
