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This detailed volume presents numerous methods used in bioengineering to create new synthetic promoters in various organisms. Additionally, the book explores mathematical models, novel screening and construction techniques, modulation of protein expression, and various applications ranging from biosensors to viral resistance. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Promoters: Methods and Protocols offers researchers a guide to the latest advances in this branch of synthetic biology.
Inhaltsverzeichnis
Chemical Reaction Models in Synthetic Promoter Design in Bacteria.- Promoter Prediction in Agrobacterium tumefaciens Strain C58 by Using Artificial Intelligence Strategies.- Promoters and Synthetic Promoters in Trichoderma reesei.- Rational Design and Screening of Synthetic Promoters in Chlamydomonas reinhardtii.- Simultaneous Plate-Reader Characterization of Promoter Activity and Cell Growth in Engineered Mammalian Cells.- Synthetic Promoter Design and Functional Evaluation in Saccharomyces cerevisiae.- Hybrid Synthetic Promoters in Saccharomyces cerevisiae Built on Foreign Promoter Sequences.- CRISPR/Cas9-Mediated Promoter Engineering in Saccharopolyspora erythraea.- Optimizing Rice Genomics: Employing the Hypercompact Cas12j2 System for Targeted Transcriptional Regulation and Epigenome Modification.- Exploring the Function of Gene Promoter Regulatory Elements Using CRISPR Tools.- Promoters in Pichia pastoris: A Toolbox for Fine-Tuned Gene Expression.- Fine-Tuning Gene Expression in Bacteria by Synthetic Promoters.- Tuning of Protein Expression by Promoter Engineering.- TED: Enhancing Translation Efficiency in Bacterial Expression Systems.- Development of a Haa1-Based Biosensor for Acetic Acid Sensing in Saccharomyces cerevisiae.- Harnessing Viral Promoters for Targeted RNAi: Engineering Phloem-Specific Resistance Against Tomato Yellow Leaf Curl Thailand Virus.- Genetic Engineering of Microorganisms with Electroactive Genes for the Fabrication of Electrochemical Microbial Biosensors.- Construction and Characterization of Light-Responsive Transcriptional Systems.
