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This volume details procedures relevant to all disciplines, with specific emphasis on challenging aspects of working with Bacterial Extracellular Vesicle (BEVs). Chapters are divided into four parts focusing on characterization of Bacterial Extracellular Vesicles, assessment of Bacterial Extracellular Vesicle Biological Function In Vitro, preparation and functionalization of therapeutic Bacterial Extracellular Vesicles, and delivery and tracking of Bacterial Extracellular Vesicles In Vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Extracellular Vesicles: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis
Methods to Analyze the Surface Composition of Bacterial Membrane Vesicles.- Analysis of Bacterial Extracellular Vesicles by Immunogold Transmission Electron Microscopy.- Methods for the Characterization of the Colloidal Properties of Bacterial Membrane Vesicles.- Methods for Bioinformatic Prediction of Genuine sRNAs from Outer Membrane Vesicles.- Screening for Genes Involved in Outer Membrane Vesicle Biogenesis in Bacteroides thetaiotaomicron.- Use of Synchrotron Radiation Circular Dichroism to Analyze the Interaction and Insertion of Proteins into Bacterial Outer Membrane Vesicles.- Methods for Assessing Plant Immune System Activation by Bacterial Extracellular Vesicles and Other Elicitors.- Methods for Studying Fusion of Bacterial Extracellular Vesicles with Intact Bacteria and Host Cells.- Multiple Particle Tracking: A Method for Probing Biologically Relevant Mobility of Bacterial Extracellular Vesicles.- Separation of Bacterial Extracellular Vesicles Via High Performance Anion Exchange Chromatography.- Spray Drying of Bacterial Membrane Vesicles for Vaccine Delivery.- Engineering Outer Membrane Vesicles to Carry Enzymes: Encapsulation, Isolation, Characterization, and Modification.- On-Demand Vaccine Production Via Dock-and-Display of Biotinylated Antigens on Bacterial Extracellular Vesicles.- Dose Determination and Administration of Bacterial Extracellular Vesicles for In Vivo Preclinical Studies.- Assessing In Vivo Bacterial Extracellular Vesicle Biodistribution Using Fluorescent Lipophilic Membrane Stains.- Molecular approaches to measure bacterial extracellular vesicles in tissue samples.
