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This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.
Inhaltsverzeichnis
From olive oil emulsions to COVID-19 Vaccines - Liposomes Came First.- Preparation of DRV liposomes.- Preparation of small unilamellar vesicles using detergent dialysis method.- Thin-film hydration followed by extrusion method for liposome preparation.- Ethanol injection method for liposome preparation.- Preparation of giant vesicles with mixed single-tail and double-tail lipids.- Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing.- Preparation of doxorubicin liposomes by remote loading method.- Magnetic thermosensitive liposomes loaded with Doxorubicin.- Preparation and physical characterization of DNA binding cationic liposomes.- Tunable pH sensitive lipoplexes.- Solid Lipid Nanoparticles for Drug Delivery.- Stable Discoidal Bicelles - Formulation, Characterization and Functions .- The post-insertion method for the preparation of PEGylated liposomes.- Click chemistry for liposome surface modification.- Surface Modification of Liposomes Using Folic Acid.- Preparation and Characterization of Trastuzumab Fab conjugated liposomes (immunoliposomes).- Pyrophosphorylated cholesterol modified bone-targeting liposomes formulation procedure.- Method of simultaneous analysis of liposome components using HPTLC/FID.- HPLC-MS/MS Method for Identification and Quantification of Lipids in Liposomes.- DPH probe method for liposome-membrane fluidity determination.- Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy.- Visualization and characterization of liposomes by Atomic Force Microscopy (AFM).- Determination of the sub-cellular distribution of fluorescently labeled liposomes using confocal microscopy.- Liposome biodistribution via europium complexes.- Quantification of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue: application to study liposome's uptake by human placenta.
