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Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications critically evaluates the basic concepts and methodologies of conventional biomarkers as well as current state-of-the-art assays for measuring antioxidant activity/oxidative stress and their practical applications. . Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications will be of a great interest to scientists who are involved in basic research on oxidation, applied scientists evaluating the effects of nutraceuticals or pharmaceutical compounds on antioxidant activity/oxidative stress, and physicians who want to understand the degree of oxidative damage in patients with certain chronic diseases.
Discovering sensitive and specific biomarkers for systemic oxidative damage is essential to understand the role of oxidative stress in human disease. Once these roles are clearly understood, we are able to identify novel drug and nutraceutical targets. This volume goes beyond conventional analytical methods of measuring overall antioxidant activity and provides insight to the discovery of biomarkers that reveal information on specific areas of oxidative stress. Contributed by an international list of experts, Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications describes both conventional biomarkers and recent developments in this area.
Special Features:
* Discusses conventional biomarkers as well as recent advances for measuring antioxidants and oxidative stress
* Biomarkers for lipid peroxidation: isoprostane, hydroxyloctadecaenoic acid, oxysterols, and reactive carbonyl species from lipid peroxidation
* Biomarkers for protein oxidation: carbonylation, tyrosine oxidation, ubiquitin-conjugation
* Biomarkers for DNA oxidative damage: comet assay, hydroxylated nucleotides, and exocylcic DNA adducts
* Recently developed biomarkers from cutting-edge technology
Inhaltsverzeichnis
PART I. Biomarkers for Antioxidant Activity.
1. Antioxidant activity and oxidative stress : An Overview.
2. Hydrophilic approach to analyze antioxidant activity.
3. Lipophilic approach to analyze antioxidant activity.
1. LDL Oxidation.
PART II. Biomarkers for Oxidative Stress Using Lipid Peroxidation Breakdown.
Products & Metabolites.
1. Arachidnoic acid peroxidation products, Isoprostanes.
2. Docosahexaenoic acid peroxidation products, neuroprostanes, and neurofurans.
3. Linoleic acid oxidation products, Hydroxyoctadecadienoic acid and oxidatively modified peroxiredoxins.
4. Cholesterol oxidation products, oxysterols.
5. GSH dependent metabolism of nucleophilic lipid peroxidation byproducts.
PART III. Biomarkers for Oxidative Stress Using Protein carbonyls.
1. Reactive carbonyl species and protein carbonylation: an overview.
2. Immunochemical detection of cabonylated proteins.
3. Mass spectrometric strategies for identification of peptides and proteins modified by reactive carbonyl species.
4. Redox proteomics identification of carbonylated proteins.
5. Ubiquitin conjugates.
PART IV. Biomarkers for Oxidative Stress Using DNA damage.
1. Single Cell Gel Electrophoresis, COMET assay.
2. Hydroxyltaed DNA bases.
3. Exocylic DNA adducts.
4. Micro DNA array.
PART V. Biomarkers for Oxidative Stress Using Oxidative Modifications of Protein.
Thiols.
1.Oxidative modifications of protein thiols and their measurements: an overview.
2. Albumin cys34 oxidation and cytseinliation.
3. Protein S-glutathionilation and S-cysteinilation.
Über den Autor / die Autorin
Giancarlo Aldini, Ph.D., Department of Pharmaceutical Sciences "Pietro Pratesi", Università degli Studi di Milano, Milan, Italy
Kyung-Jin Yeum, Ph.D., Jean Mayer USDA-Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA.
Etsuo Niki, Ph.D., , Health Research Institute, National Institute of Advanced Industrial Science & Technology, Osaka, Japan.
Robert M. Russell, M.D., Office of Dietary Supplements, National Institutes of Health, 6100 Executive Blvd., Bethesda, MD 20892-7517, USA
Zusammenfassung
Discovering sensitive and specific biomarkers for systemic oxidative damage is essential to better understand the role of oxidative stress in human disease. Once these roles are more clearly understood we are able to identify novel drug and nutraceutical targets.
Bericht
"Chemists, pharmacologists, and other researchers describe theprinciples behind current methods for measuring antioxidantactivity and oxidative stress and their practical applications inspecific health situations." ( SciTech Book News, December2010)
