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A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.
Inhaltsverzeichnis
Forensic DNA-Typing Technologies.- DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR.- A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples.- Species Determination.- Constructing STR Multiplex Assays.- Y Chromosome STR Typing.- Using Online Databases for Developing SNP Markers of Forensic Interest.- SNP Typing in Forensic Genetics.- Genotyping SNPs With the LightCycler.- Universal Tag Arrays in Forensic SNP Analysis.- SNP Typing on the NanoChip Electronic Microarray.- Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS.- Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing.- SNaPshot Typing of Mitochondrial DNA Coding Region Variants.- Typing of Y Chromosome SNPs With Multiplex PCR Methods.- Y Chromosome SNP Analysis Using the Single-Base Extension.- SNaPshot for Pharmacogenetics by Minisequencing.- Methods for the Study of Ancient DNA.- Protocols for Ancient DNA Typing.
Zusammenfassung
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.
