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Informationen zum Autor CHARLES L. WILKINS , PhD, is a Distinguished Professor in the Department of Chemistry and Biochemistry at the University of Arkansas. He is the author or coauthor of over 250 scientific publications and has received numerous awards for his research. JACKSON O. LAY, Jr. , PhD, is the Director of the Arkansas Statewide Mass Spectrometry Facility at the University of Arkansas, and the author or coauthor of more than 100 publications in the field. In addition to his work with bacteria, he has developed mass spectrometry methods for cancer research and toxicology. Klappentext A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysisFrom chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications.Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including:* Metastable atom bombardment pyrolysis mass spectrometry* Matrix-assisted laser desorption/ionization mass spectrometry (MALDI)* MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria* High-resolution Fourier transform mass spectrometry (FTMS)* Electrospray ionization (ESI) mass spectrometryIdentification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data. Zusammenfassung Identification of Microorganism by Mass Spectrometry teaches the reader how to use the latest techniques in mass spectrometry to identify the presence of microorganisms for biochemical, biomedical, pharmaceutical, and bioterror applications. Inhaltsverzeichnis Preface. Contributors. 1. Cultural, Serological and Genetic Methods for Identification of Bacteria (John B. Sutherland and Fatemeh Rafii). 1.1 Introduction. 1.2 Identification of bacteria by cultural methods. 1.3 Identification of bacteria by serological methods. 1.4 Identification of bacteria by genetic methods. 1.5 Other methods used for bacterial characterization. 1.6 Conclusions. 1.7 Acknowledgments and Disclaimers. 1.8 References. 2. Mass Spectrometry: Identification and Biodetection, Lessons Learned and Future Developments (Alvin Fox). 2.1 Introduction. 2.1.1 Analysis of Fatty Acid and Sugar Monomers using GC-FID, GC-MS and GC-MS-MS. 2.1.2 2.2 Analysis of PCR products using PCR, PCR-MS and PCR-MS-MS. 2.1.3 Analysis of proteins using MALDI-TOF MS. 2.1.4 Chemical markers for protein-based identification or biodetection. 2.1.5 Conclusions. 2.1.6 References. 3. An Introduction to Maldi TOF MS Analysis of Whole Bacteria (Rohana Liyanage and Jackson O. Lay, Jr.). 3.1. Introduction. 3.2. Mass Spectrometry and Time-of-Flight MS. 3.3. Matrix Assisted Laser Desorption Ionization. <...
