Neural Stem Cells: - No Rights This Territory

Klappentext Over the last decade, neural stem cell research has provided penetrating insights into the plasticity and regenerative potential of the brain. Stem cells have been isolated from embryonic as well as adult central nervous system (CNS). Many non-CNS mammalian tissues also contain stem cells with a more limited repertoire: the replacement of tissue-specific cells throughout the li- time of the organism. Progress has been made in understanding fundamental stem cell properties that depend on the interplay of extrinsic signaling factors with intrinsic genetic programs within critical time frames. With this growing knowledge, scientists have been able to change a neural stem cell's fate. - der certain conditions, neural stem cells have been induced to differentiate into cells outside the expected neural lineage and conversely, stem cells from nonneural tissue have been shown to transdifferentiate into cells with distinct neural phenotypes. At the moment, there is an accelerated effort to identify a readily ava- able, socially acceptable stem cell that can be induced to proliferate in an und- ferentiated state and that can be manipulated at will to generate diverse cells types. We are on the threshold of a great new therapeutic era of cellular therapy that has as great, if not greater, potential as the current pharmacologic era, g- rified by antibiotics, anesthetics, pain killers, immunosuppressants, and psyc- tropics. Inhaltsverzeichnis Part I. Isolation and Culture of NSCs Neural Differentiation of Embryonic Stem Cells K. Sue O'Shea Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, Tong Zheng, and Dennis A. Steindler Isolation of Stem and Precursor Cells from Fetal Tissue Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb Susan C. Barnett and A. Jane Roskams Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium Edmund Au and A. Jane Roskams Production of Immortalized Human Neural Crest Stem Cells Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, Myung A. Lee, and Jung H. Bang Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization Lamya S. Shihabuddin Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood Shijie Song and J. Sanchez-Ramos Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer Part II. Characterization of NSCs In Vitro A. Cellular Techniques Analysis of Cell Generation in the Telencephalic Neuroepithelium Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures Angelo L. Vescovi, Rossella Galli, and Angela Gritti Assessing the Involvement of Telomerase in Stem Cell Biology Mark P. Mattson, Peisu Zhang, and Weiming Fu Detection of Telomerase Activity in Neural Cells Karen R. Prowse In Vitro Assays for Neural Stem Cell Differentiation Marcel M. Daadi Electron Microscopy and Lac-Z Labeling Bela Kosaras and Evan Snyder B. Electrophysiological Techniques Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells David S. K. Magnuson and Dante J. Morassutti C. Molecular Techniques Fluorescence In Situ Hybridization Barbara A. Tate and Rachel L. Ostroff RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells Theresa E. Gratsch Differential Display: Isolation of Novel Genes Theresa E. Gratsch Cell Labeling and Gene Misexpression by Electroporation Terence J. Van Raay and Michael R. Stark Gene Therapy Using Neural Stem Cells Luciano Conti and Elena Cattaneo Modeling Brain Pathologies Using Neural Stem Cells Simonetta Sipione...

Produktdetails

• Autoren: Paul R. Sanberg, J Sanchez, Juan R. Sanchez-Ramos, Tanja Zigova
• Verlag: Humana Press
• Sprache: Englisch
• Produktform: Fester Einband
• Erschienen: 15.09.2002
• EAN: 9780896039643
• ISBN: 978-0-89603-964-3
• Serie: Awakening the Word Series
• Thema: Naturwissenschaften, Medizin, Informatik, Technik > Medizin