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Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo.
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
Inhaltsverzeichnis
Delivery Using Chemical Methods.- Chemical Methods for DNA Delivery.- Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran.- DNA Delivery to Cells in Culture Using Peptides.- DNA Delivery to Cells in Culture Using PNA Clamps.- Dendrimer-Mediated Cell Transfection in Vitro.- DNA Delivery to Cells in Culture Using Cationic Liposomes.- Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium.- Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium.- Delivery of DNA to Tumor Cells Using Cationic Liposomes.- Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes.- Delivery Using Physical Methods.- Gene Delivery Using Physical Methods.- Gene Delivery to Mammalian Cells by Microinjection.- Delivery of DNA to Cells in Culture Using Particle Bombardment.- Delivery of DNA to Skin by Particle Bombardment.- Biolistic Transfection of Cultured Organotypic Brain Slices.- Efficient Electroporation of Mammalian Cells in Culture.- Delivery of DNA to Skin by Electroporation.- In Vivo DNA Electransfer in Skeletal Muscle.- Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo.- Hydrodynamic Delivery of DNA.- Naked DNA Gene Transfer in Mammalian Cells.- Microparticle Delivery of Plasmid DNA to Mammalian Cells.- DNA Delivery to Cells in Culture Using Ultrasound.- DNA Delivery to Cells In Vivo by Ultrasound.
Zusammenfassung
Describes in step-by-step detail methods that have proven useful in delivering genes to mammalian cells. This title focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics.
Zusatztext
For Volumes 1 & 2
"This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology
Volume 1
"...provides insights into the choices available in this important area of biology and medicine." - Microbiology Today
Bericht
For Volumes 1 & 2
"This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology
Volume 1
"...provides insights into the choices available in this important area of biology and medicine." - Microbiology Today
