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This volume details the impact of aging on biological system and to understand the basis of this diversity with the goal of elucidating mechanisms to optimize health. Chapters guide readers through different animal models and methodologies to examine aging at the organism, tissue, cellular, and molecular levels across the lifespan. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Aging: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis
Measuring the Replicative Lifespan of Saccharomyces cerevisiae using the HYAA Microfluidic Platform.- Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches.- Simple Culture Apparatus for Individual C. elegans Culture.- Analysis of Drosophila melanogaster Life Span.- Assessment of Mouse Fitness as Determined through Treadmill Running and Walking.- Human Population Genetics in Aging Studies for Molecular Biologists.- Design and Analysis of Pharmacological Studies in Aging.- Methods for Assessing Fertility in C. elegans from a Single Population.- Metabolic Assessment of Lipid Abundance and Distribution.- Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography-Mass Spectrometry.- Assessing Insulin and Glucose Tolerance in the Aging Mouse.- High-throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome.- Measurements of Innate Immune Function in C. elegans.- Measuring Phagocytosis in Bone Marrow-Derived Macrophages and Peritoneal Macrophages with Aging.- Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells.- Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age.- Measuring Tissue-specific Autophagy Flux in Adult Caenorhabditis elegans.- Assay Development and Measurement of the Aging Biomarker Humanin.- Staining and Quantification of beta-amyloid Pathology in Transgenic Mouse Models of Alzheimer's Disease.- Multimodal Imaging of Cerebral Microhemorrhages and White Matter Degradation in Geriatric Patients with Mild Traumatic Brain Injury.- Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-based Approach.- Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging.
