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This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and diseased human specimens; methods to evaluate specific PTEN function in brain cancer; methods that utilize a new biosensor to measure PTEN regulation; and techniques to measure post-transcriptional regulation of PTEN by micoRNAs and ceRNAs. Other chapters present methods describing novel techniques to detect PTEN localization and previously unstudied structural features of PTEN measured through X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry. The book concludes with methods to study PTEN function in model organisms including mice and C. elegans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, PTEN: Methods and Protocols is a valuable collection of methodologies and protocols useful to researchers who are interested in the PTEN field.
Inhaltsverzeichnis
PTEN: History of a Tumor Suppressor.- PTEN at 18: Still Growing.- Immunopathologic Assessment of PTEN Expression.- Measurement of PTEN by Flow Cytometry.- Detecting PTEN and PI3K Signalling in Brain.- Germline PTEN Mutation Analysis for PTEN Hamartoma Tumor Syndrome.- Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis.- Rapid Detection of Dynamic PTEN Regulation in Living Cells Using Intramolecular BRET.- Methods for the Identification of PTEN-Targeting MicroRNAs.- Post-Transcriptional Regulation of PTEN by Competing Endogenous RNAs.- In Cell and In Vitro Assays to Measure PTEN Ubiquitination.- Assessing PTEN Subcellular Localization.- Methods to Study PTEN in Mitochondria and Endoplasmic Reticulum.- Methods in the Study of PTEN Structure: X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry.- Methods for PTEN in Stem Cells and Cancer Stem Cells.- Modeling Cancer Associated Mutations of PTEN in Mice.- C. elegans Methods to Study PTEN.
Über den Autor / die Autorin
Leonardo Salmena Ph.D. Assistant Scientist Ontario Cancer Institute/University Health Network Princess Margaret Hospital 9-412 610 University Avenue Toronto, Ontario M5G 2M9 E: salmenal@uhnres.utoronto.ca P: 416-946-4501 Ext. 3695 Vuk Stambolic, PhD Senior Scientist Ontario Cancer Institute (OCI) Primary Office Princess Margaret Cancer Centre 10th Floor Rm 10-123 610 University Ave Toronto, Ontario Canada M5G 2M9
Zusammenfassung
This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology.
