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Klappentext The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. In Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition this important area of research is brought up-to-date by the leading scientists in the field. This compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Each chapter provides a brief introduction to the topic, step-by-step laboratory protocols, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Post-Translational Modifications of Proteins: Tools for Functional Proteomics, Third Edition serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries. Zusammenfassung The majority of all proteins undergo co- and/or post-translational modifications, crucially altering physical and chemical properties, folding, conformation distribution, stability, activity, and, consequently, the function of the proteins. Inhaltsverzeichnis Web-Based Computational Tools for the Prediction and Analysis of Post-Translational Modifications of Proteins.- Detection of Post-Translational Modifications by Fluorescent Staining of Two-Dimensional Gels.- Analysis of Ser/Thr Linked Sugar Chains.- Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling.- Enzymatic Sequence Analysis of N-Glycans By Exoglycosidase Cleavage and Mass Spectrometry: Detection of Lewis X Structures.- Chromatographic Profiling of N-Glycans.- Activity Determination of Glycosyltransferases.- Characterization of Site-Specific N-Glycosylation.- Glycosylation of Type I Collagen.- Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa.- Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods.- Mass Spectrometry-Based Method for Detection and Identification of Free Thiol Groups in Proteins.- Mass Spectrometric Determination of Protein Ubiquitination.- Analysis of Sumoylation.- An Approach for the Identification of Proteins Modified by ISG15.- a-Amidated Peptides: Approaches for Analysis.- S-Acylation of Proteins.- Analysis of Methylation, Acetylation, and Other Modifications in Bacterial Ribosomal Proteins.- Lysine Hydroxylation and Cross-Linking of Collagen. ...
