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This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detail current status and perspectives of transgenic cotton, principle and methods for making transgenic cotton, creating gene knockout lines, foreign gene copy and expression in transgenic plants, improvements to cotton using transgenic technology, and monitoring the potential impact of transgenic cotton on environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Authoritative and cutting-edge, Transgenic Cotton: Methods and Protocols 2nd aims to be a resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology, and agricultural sciences.
Inhaltsverzeichnis
Transgenic Cotton: From Biotransformation Methods to Agricultural Application.- Agrobacterium-mediated Genetic Transformation of Cotton.- Biolistic Transformation of Cotton Zygotic Embryo Meristem.- Novel Pollen Magnetofection System for Transformation of Cotton Plant with Magnetic Nanoparticles as Gene Carriers.- Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures.- Pollen Tube Pathway-Mediated Cotton Transformation.- Embryogenic Calli Explants and Silicon Carbide Whisker-mediated Transformation of Cotton (Gossypium hirsutum L.).- Genome Editing in Cotton Using CRISPR/Cas9 System.- Tobacco Rattle Virus Induced Gene Silencing in Cotton.- Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome.- Estimating the Copy Number of Transgenes in Transformed Cotton by Real-time Quantitative PCR.- Development of an Enzyme-linked Immunosorbant (ELISA) Assay for the Detection of GM Protein in GM Crops/Produce.- Screening of Transgenic Cotton Based on a Porous Silicon Biosensor.- YC3.60-based Imaging Analysis on Calcium Level in Cotton Cells.- A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene.- A Grafting Technique for Efficiently Transplanting Transgenic Regenerated Plants of Cotton.- Inheritance of Transgenes in Transgenic Bt lines Resistance to Helicoerpa armigera in Upland Cotton.- Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics.- Overexpression of miRNA in Cotton via Agrobacterium-mediated Transformation.- Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover.- Next Generation Transgenic Cotton: Pyramiding RNAi with Bt Counters Insect Resistance.- Genetic Transformation of Cotton with the Harpin-encoding Gene hpaXoo of Xanthomonas oryzae pv. Oryzae and Evaluation of Resistance against Verticillium Wilt.- Development of Insect-resistant Transgenic Cotton with Chimeric TVip3A* Accumulating in Chloroplasts.- Development of Virus Resistance Transgenic Cotton using Cotton Leaf Curl Virus Antisense ßC1 Gene.- Determining Pollen-mediated Gene Flow in Transgenic Cotton.
Zusammenfassung
This second edition provides a comprehensive collection of the cutting-edge methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Chapters detail current status and perspectives of transgenic cotton, principle and methods for making transgenic cotton, creating gene knockout lines, foreign gene copy and expression in transgenic plants, improvements to cotton using transgenic technology, and monitoring the potential impact of transgenic cotton on environment. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Authoritative and cutting-edge, Transgenic Cotton: Methods and Protocols 2
nd
aims to be a resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology, and agricultural sciences.
