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Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.
Inhaltsverzeichnis
Visualizing Wnt Palmiteoylation in Single Cells.- Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction.- Biochemical Methods to Analyze Wnt Protein Secretion.- Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum.- Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt Pathway.- A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in Cells.- Wnt-Dependent Control of Cell Polarity in Cultured Cells.- The Use of Chick Embryos to Study Wnt Signaling/Gradients.- Monitoring Wnt Signaling in Zebrafish Using Fluorescent Biosensors.- Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In Vivo.- Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg Extracts.- Delivery of the Porcupine Inhibitor WNT974 in Mice.- Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in Osteogenesis.- Monitoring Wnt/b-Catenin Signaling in Skin.- The Generation of Organoids for Studying Wnt Signaling.- Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem Cells.- Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.
Zusammenfassung
Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study.
