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This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis
Improved denaturation of Small RNA Duplexes and its Application for Northern-blotting.- High throughput RT-qPCR for the Analysis of Circulating microRNAs.- Genome-wide Comparison of Next Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum.- Small RNA Profiling by Next Generation Sequencing using High Definition Adapters.- Surface Acoustic Wave Lysis and Lon-exchange Membrane Quantification of Exosomal microRNA.- Droplet Microfluidic Device Fabrication and use for Isothermal Amplification and Detection of microRNA.- Interrogation of Functional miRNA-target Interactions by CRISPR/Cas9 Genome Engineering.- Cell-free Urinary microRNAs Expression in small-scale Experiments.- Peptide-based Isolation of Argonaute Protein Complexes using Ago-APP.- Predicting functional microRNA-mRNA interactions.-Computational and Experimental Identification of Tissue-specific microRNA Targets.- sRNAtoolboxVM: Small RNA Analysis in a Virtual Machine.- An Assessment of the Next Generation of Animal miRNA Target Prediction Algorithms.- The UEA Small RNA Workbench: A Suite of Computational Tools for Small RNA Analysis.- Prediction of miRNA-mRNA Interactions using miRGate.- Detection of microRNAs using Chip-based QuantStudio 3D Digital PCR.- MiRNA Quantitation with Microelectrode Sensors Enabled by Enzymeless Electrochemical Signal Amplification.- A Robust Protocol to Quantify Circulating Cancer Biomarker microRNAs.- MicroRNAs, Regulatory Networks and Comorbidities: Decoding Complex Systems.- Label-free Direct Detection of MiRNAs with Poly-silicon Nanowire Biosensors.
Zusammenfassung
This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization.
