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This volume presents methods and techniques to studyoogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guidesreaders through protocols on models ofdevelopmental biology, oogenesis in plants, worms, fruit flies,mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritative and cutting-edge, Oogenesis:Methods and Protocols aims to ensure successful results in the furtherstudy of this vital field.
Inhaltsverzeichnis
Histological Analysis of the ArabidopsisGynoecium and Ovules Using Chloral Hydrate Clearing and Differential InterferenceContrast Light Microscopy.- Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis Elegans.- Antibody Staining In Drosophila Germaria.- In VitroDrosophilaEgg Chambers: A History and Adaptable Method.- Using Fluorescent Reporters to Monitor Autophagy in the Female Germ Line Cellsin Drosophila Melanogaster.- Analysis of Phagocytosis in the Drosophila Ovary.- Immuno-Gold Labeling Of Drosophila FolliclesFor Transmission Electron Microscopy.- Preparation of Drosophila Follicles forTransmission Electron Microscopy.- Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles.- Studying Oogenesis in a Non-Model Organism Using Transcriptomics; Assembling,Annotating, and Analysing your Data.- Live Imaging ofCentriole Dynamics by Fluorescently-Tagged Proteins un Starfish Oocyte Meiosis.- High-Pressure Freezing Electron Microscopy ofZebrafish Oocytes.- Cytoskeleton and Cytoskeleton-Bound RNA Visualizationin Frog and Insect Oocytes.- RNA FISH, DNA FISH and Chromosome Painting ofChicken Oocytes.- Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis.- Quantitative Microinjection of Morpholino Antisense Oligonucleotides into MouseOocytes to Examine Gene Function in Meiosis-I.- Monitoring Calcium Oscillations in FertilizedMouse Eggs.- Live Imaging of Intracellular Dynamics During Meiotic Maturation in MouseOocytes.- Isolation of Mammalian Oogonial Stem Cells by Antibody-Based FluorescenceActivated Cell Sorting.- Immunohistochemistry of Paraffin Sections from Mouse Ovaries.
Zusammenfassung
This volume presents methods and techniques to study
oogenesis in a broad range of organisms, from plants to mammals.
Oogenesis: Methods and Protocols
guides
readers through protocols on models of
developmental biology, oogenesis in plants, worms, fruit flies,
mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful
Methods
in Molecular Biology
series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and cutting-edge,
Oogenesis:
Methods and Protocols
aims to ensure successful results in the further
study of this vital field.
