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This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in Cell Cycle Synchronization: Methods and Protocols, Second Edition cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Cell Cycle Synchronization: Methods and Protocols, Second Edition is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, computer science, and life sciences. Specialists and professionals in cell biology, genetics, molecular biology, biochemistry, and pharmacology will also find this book useful.
Inhaltsverzeichnis
Overview of Cell Synchronization.- Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation.- Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Cells.- Large Scale Mitotic Cell Synchronization.- Synchronization and Desynchronization of Cells by Interventions on the Spindle Assembly Checkpoint.- Synchronization of Mammalian Cell Cultures by Serum Deprivation.- DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry.- Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias Latipes.- Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate.- Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture.- Analysis of Nuclear Uracil DNA - Glycosylase (nUDG) Turnover During the Cell Cycle.- Synchronization of Hela Cells.- Synchronization of Bacillus Subtilis Cells by Spore Germination and Outgrowth.- Synchronization of Yeast.- Synchronization of Pathogenic Protozoans.- Synchronization of In Vitro Maturation in Porcine Oocytes.- Detection of Changes in the Medicago sativa Retinoblastoma-Related Protein (MsRBR1) Phosphorylation during Cell Cycle Progression in Synchronized Cell Suspension Culture.- Nuclear Treatment and Cell Cycle Synchronization for the Purpose of Mammalian and Primate Somatic Cell Nuclear Transfer (SCNT).- Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation.- Intracellular Flow Cytometry Improvements in Clinical Studies.- Molecular Network Dynamics of Cell Cycle Control: Periodicity of Start and Finish.
Zusammenfassung
This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques.
