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Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.
Inhaltsverzeichnis
Applications of Molecular Genetics to the Study of Asthma.- Assessment and Validation of New Genetic Variants: A systematic In Silico Approach.- Introduction to the Gene Expression Analysis.- Real Time PCR for Gene Expression Quantification in Asthma.- Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma.- Review of Methods to Study Gene Expression Regulation Applied to Asthma.- Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma.- SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma.- Chromatin Immunoprecipitation: Application to the Study of Asthma.- Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols.- Protocols for Exosome Isolation and RNA Profiling.- Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell.- Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line.- Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.- Review of Mouse Models Applied to the Study of Asthma.- General and Specific Mouse Models for Asthma Research.- Mouse Models applied to the Research of Pharmacological Treatments in Asthma.- Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.
Zusammenfassung
Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.
