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The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.
Inhaltsverzeichnis
Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa.- Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5' End Maturation.- Mitochondrial Run-On Transcription Assay Using Biotin Labeling.- In Vitro RNA Uptake Studies in Plant Mitochondria.- In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria.- Plant Mitochondrial Proteomics.- Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites.- A Flowchart to Analyze Protease Activity in Plant Mitochondria.- Activity Measurements of Mitochondrial Enzymes in Native Gels.- Activity Assay for Plant Mitochondrial Enzymes.- Analysis of Type II NAD(P)H Dehydrogenases.- Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes.- Micro-Respiration Measurements in Plants.- Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation.- Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana.- Imaging and Analysis of Mitochondrial Dynamics in Living Cells.- Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors.- In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide.- Databases and Informatics Resources For Analysis of Plant Mitochondria.- Expression and Crystallization of the Plant Alternative Oxidase.
Zusammenfassung
The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities.
