Mitochondrial Medicine - Volume II, Manipulating Mitochondrial Function

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Mitochondrial Medicine provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

Inhaltsverzeichnis

DQAsomes as the Prototype of Mitochondria-targeted Pharmaceutical Nanocarriers: Preparation, Characterization, and Use.- Predicting Mitochondrial Targeting by Small Molecule Xenobiotics Within Living Cells Using QSAR Models.- Targeting Mitochondria with Small Molecules: The Preparation of MitoB and MitoP as Exomarkers of Mitochondrial Hydrogen Peroxide.- Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes.- Synthesis and Evaluation of ¹⁸F-Labeled Fluoroalkyl Triphenylphosphonium Salts as Mitochondrial Voltage Sensors in PET Myocardial Imaging.- Bridging the Gap Between Nature and Antioxidant Setbacks: Delivering Caffeic Acid to Mitochondria.- Mitochondriotropic Nano-Emulsified Genistein-Loaded Vehicles for Cancer Therapy.- Formulation and Optimization of Mitochondria-Targeted Polymeric Nanoparticles.- Ex Vivo Generation of Functional Immune Cells by Mitochondria-Targeted Photosensitization of Cancer Cells.- Targeting the Mitochondrial Genome Via a Dual Function MITO-Porter: Evaluation of mtDNA-Levels and Mitochondrial Function.- Mitochondrial, Acidic, and Cytosolic pHs Determination by ³¹P NMR Spectroscopy: Design of New Sensitive Targeted pH Probes.- Methodology for Use of Mitochondria-Targeted Cations in the Field of Oxidative Stress-Related Research.- Synthesis and Testing of Novel Isomeric Mitochondriotropic Derivatives of Resveratrol and Quercetin.- Evaluation of Respiration of Mitochondria in Cancer Cells Exposed to Mitochondria-Targeted Agents.- The Effect of Mitochondrially Targeted Anti-Cancer Agents on Mitochondrial (Super)Complexes.- Mitochondrial Targeting of Recombinant RNA.- Mitochondrial Targeting of Catalytic RNAs.- Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease.- Analysis of Pollutant-InducedChanges in Mitochondrial DNA Methylation.- ETS and DEAS Studies of the Reduction of Xenobiotics in Mitochondrial Intermembrane Space.- Analysis of Mitochondrial Morphology and Function Under Conditions of Mitofusin 2 Deficiency.- Yeast as a Tool to Study Mitochondrial Retrograde Pathway En Route to Cell Stress Response.- Cell Energy Budget Platform for Assessment of Cell Metabolism.- Induced Pluripotent Stem Cells (iPSCs) for Modeling Mitochondrial DNA Disorders.- Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice.- In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans.- Mitochondrial DNA as a Biosensor of UV Exposure in Human Skin.- Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation.- Atomic Force Microscopy-Based Shape Analysis of Heart Mitochondria.- Enzymatic Assays for Probing Mitochondrial Apoptosis.- A Cybrid Cell Model for the Assessment of the Link Between Mitochondrial Deficits and Sporadic Parkinson's Disease.- Analysis of Mitochondrial Network by Imaging: Proof of Technique in Schizophrenia.

Zusammenfassung

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Mitochondrial Medicine provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

Produktdetails

• Mitarbeit: Edeas (Herausgeber), Edeas (Herausgeber), Marvin Edeas (Herausgeber), Volkma Weissig (Herausgeber), Volkmar Weissig (Herausgeber)
• Verlag: Springer, Berlin
• Sprache: Englisch
• Produktform: Taschenbuch
• Erschienen: 01.01.2016
• EAN: 9781493956050
• ISBN: 978-1-4939-5605-0
• Seiten: 447
• Abmessung: 179 mm x 256 mm x 18 mm
• Gewicht: 995 g
• Illustration: XXIII, 447 p. 124 illus., 65 illus. in color.
• Serien: Methods in Molecular Biology; Methods in Molecular Biology
• Thema: Naturwissenschaften, Medizin, Informatik, Technik > Medizin > Pharmazie