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The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytological manipulations, the production of synthetic polyploids, and in vitro cultures and genetic modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Oat: Methods and Protocols is a useful resource in the development of new research approaches toward organizing the oat genome and the identification of new and useful traits for further improvements of this exceptional crop.
Inhaltsverzeichnis
Fluorescence In Situ Hybridization in Oat.- Oat Doubled Haploids Following Maize Pollination.- Wide Hybridization between Oat and Pearl Millet.- Protocol for Producing Synthetic Polyploid Oats.- Manipulation of Oat Protoplasts for Transient Expression Assays.- Oat Anther Culture and Use of DH-Lines for Genetic Mapping.- Agrobacterium-Mediated Transformation of Leaf Base Segments.- Chromatographic Methods to Evaluate Nutritional Quality in Oat.- Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry.- Multiplex Dipstick Immunoassay for Semi-Quantitative Determination of Fusarium Mycotoxins in Oat.- Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat.- M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm.- Genotyping-By-Sequencing and its Application to Oat Genomic Research.- Genome-Wide Association Analysis using R.- de novo Transcriptome Assembly in Polyploid Species.- Isolation of Oat (Avena sativa L.) Total Proteins and their Prolamin Fractions for 2-D Electrophoresis.- 2-DE Separation and Identification of Oat (Avena sativa L.) Proteins and their Prolamin Fractions.- Selected Bioinformatic Tools and MS (MALDI-TOF, PMF) Techniques used in the Strategy for the Identification of Oat Proteins after 2-DE.
Zusammenfassung
The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytological manipulations, the production of synthetic polyploids, and in vitro cultures and genetic modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Oat: Methods and Protocols is a useful resource in the development of new research approaches toward organizing the oat genome and the identification of new and useful traits for further improvements of this exceptional crop.
