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Informationen zum Autor PROFESSOR PAUL VERKADE, PHD, has been working in the field of Correlative Microscopy for over 15 years and is currently based at the School of Biochemistry at the University of Bristol, United Kingdom. DR LUCY COLLINSON, PHD, has been working in the field of Correlative Microscopy for the last 18 years and is currently Head of Electron Microscopy at the Francis Crick Institute, London, United Kingdom. Inhaltsverzeichnis List of Contributors xi Preface xiii 1 It's a Small, Small World: A Brief History of Biological Correlative Microscopy 1 Christopher J. Guérin, Nalan Liv, and Judith Klumperman 1.1 It All Began with Photons 1 1.2 The Electron Takes Its Place 2 1.3 Putting It Together, 1960s to 1980s 3 1.4 CLEM Matures as a Scientific Tool 1990 to 2017 4 Acknowledgments 13 References 13 2 Challenges for CLEM from a Light Microscopy Perspective 23 Kurt Anderson, Tommy Nilsson, and Julia Fernandez¿Rodriguez 2.1 Introduction 23 2.1.1 Electron and Light Microscopy 23 2.1.2 Correlative Microscopy: Two Cultures Collide 25 2.2 Microscopy Multiculturalism 26 2.2.1 When Fluorescence Light Microscopy Resolution is Not Enough 26 2.2.2 The Fluorescence Microscopy (FM), Needle/Haystack Localization 27 2.2.3 Electron Microscopy, Visualizing the Ultrastructure 27 2.2.4 Finding Coordinates 28 2.3 Bridging the Gap between Light and Electron Microscopy 29 2.3.1 Finding the Same Cell Structure in Light and Electron Microscopes 29 2.3.2 Making the Fluorescence Labels Visible in the Electron Microscope 29 2.3.3 Visualizing Membrane Trafficking Using CLEM 30 2.4 Future CLEM Applications and Modifications 31 2.4.1 Correlative Reflection Contrast Microscopy and Electron Microscopy in Tissue Sections 31 2.4.2 Dynamic and Functional Probes for CLEM 32 References 34 3 The Importance of Sample Processing for Correlative Imaging (or, Rubbish In, Rubbish Out) 37 Christopher J. Peddie and Nicole L. Schieber 3.1 Introduction 37 3.2 Searching for Correlative Electron Microscopy Utopia 40 3.3 Sample Processing for Correlative Imaging: A Primer for the First Steps 40 3.4 Making It Go Faster (We Want More Speed, More Speed...) 42 3.5 Embedding Resins 44 3.6 Keeping the Region of Interest in Sight 45 3.7 Correlation and Relocation with Dual Modality Probes 48 3.8 Integration of Imaging Modalities, and In¿Resin Fluorescence 49 3.9 Streamlining the Correlative Approaches of the Future: SmartCLEM 51 3.10 How Deep Does the Rabbit Hole Go? 52 3.11 Hold That Thought, Though ¿ Is This All Completely Necessary? 53 3.12 Improving Accessibility to Correlative Workflows 54 3.13 Coming to the End 55 References 55 4 3D CLEM: Correlating Volume Light and Electron Microscopy 67 Saskia Lippens and Eija Jokitalo 4.1 Introduction 67 4.2 Imaging in 3D 68 4.3 Comparative and Correlative LM and EM Imaging 69 4.4 CLEM is More than LM + EM 69 4.5 3D CLEM 70 4.6 Two Workflows for 3D CLEM 71 4.7 Where is CLEM Going in the Future? 74 Acknowledgments 76 References 77 5 Can Correlative Microscopy Ever Be Easy? An Array Tomography Viewpoint 81 Irina Kolotuev and Kristina D. Micheva 5.1 Introduction 81 5.2 Why Array Tomography? 81 5.3 Array Tomography of Abundant Subcellular Structures: Synapses 82 5.4 Array Tomography of Sparsely Distributed Structures: Cisternal Organelle 84 5.5 Array Tomography of Small Model Organisms: C. elegans 87 5.6 To Summarize: Finding the Right AT Approach 90 5.7 Areas of Improvement 91 5.7.1 Resin 91 5....
