Mehr lesen
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are organized intothree major parts: Part I is a section on conventional bolus gene deliveryvectors that introduces typical transfection approaches relyingon the addition of transfectants to the cell culture medium where the cells aregrown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers orstimuli-responsive polymers that change according to the environment they arein and delivered by dripping into cells; Part III discusses examples ofsubstrate-mediated gene delivery-also termed reversetransfection-and the immobilization of a gene delivery vector onto a surface asopposed to more typical bolus delivery from the medium. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods andProtocols is written for experimentalists, and is an essential part of manylaboratory bookshelves. This book will help novice and professionals alikesucceed in their research in this field.
Inhaltsverzeichnis
Physical Chemical and BiomolecularMethods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitrofor the Gene Therapy Applications.- Cationic Lipid-Based Nucleic Acid Vectors.- Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPELiposomes and Gemini Micelles.- Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA.- Elaboration and Physicochemical Characterization of Niosome-BasedNioplexes for Gene Delivery Purposes.- Quantitative Intracellular Localization of Cationic Lipid-NucleicAcid Nanoparticles with Fluorescence Microscopy.- TargetedDelivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells.- Lipoplexes Strengthened by AnionicPolymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipidsand Anionic Polymers.- Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.- PolyallylamineDerivatives: Novel Non-Toxic Transfection Agents.- BiodegradableThree-Layered Micelles and Injectable Hydrogels.- Cationic Lipid-CoatedPolyplexes ("Lipopolyplexes") for DNA and Small RNA Delivery.- Preparation of Targeted AnionicLipid-Coated Polyplexes for MicroRNA Delivery.- Characterizationand Investigation of Redox-Sensitive Liposomes for Gene Delivery.- From ArtificialAmino Acids to Sequence-Defined Targeted Oligoaminoamides.- Gene DeliveryMethod Using Photo-Responsive Poly(beta-Amino Ester) as Vectors.- Thermo-ResponsivePolyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for SystemicGene Therapy.- Application of Polyethylenimine-Grafted Silicon NanowireArrays for Gene Transfection.
Zusammenfassung
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of
non-viral gene delivery vectors. The chapters in this book are organized into
three major parts: Part I is a section on conventional bolus gene delivery
vectors that introduces typical transfection approaches relying
on the addition of transfectants to the cell culture medium where the cells are
grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or
stimuli-responsive polymers that change according to the environment they are
in and delivered by dripping into cells; Part III discusses examples of
substrate-mediated gene delivery—also termed reverse
transfection—and the immobilization of a gene delivery vector onto a surface as
opposed to more typical bolus delivery from the medium. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and
Protocols is written for experimentalists, and is an essential part of many
laboratory bookshelves. This book will help novice and professionals alike
succeed in their research in this field.
