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Informationen zum Autor Editor-in-Chief, Dr. Michael C. Flickinger is the Associate Director for Academic Programs of the Golden LEAF Biomanufacturing Training and Education Center (BTEC) and a Professor of Microbiology and Chemical and Biomolecular Engineering at North Carolina State University, Raleigh. Dr. Flickinger was Director of the University of Minnesota's Institute for Advanced Studies in Biological Process Technology, which was later renamed the Biotechnology Institute ), and a Professor of Biochemistry, Molecular Biology and Biophysics at the University of Minnesota, St. Paul. Prior to coming to Minnesota Dr. Flickinger was Director of the Fermentation Program at the National Cancer Institute in Frederick, Maryland. Dr. Flickinger received his academic training at the University of Wisconsin, Madison.?(B.S.?(1973) in microbial biochemistry;? M.S. (1975) and Ph.D.?(1977) in pharmaceutical biochemistry.)? He did his postdoctoral work at the Laboratory for Renewable Resources Engineering? in the School of Chemical Engineering, Purdue University. He is Editor-in-Chief of the Encyclopedia of Industrial Biotechnology (Wiley, 2010) and Co-Editor of the Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (Wiley, 1999). Klappentext Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. This guide to biomanufacturing contains carefully selected articles from Wiley's Encyclopedia of Industrial Biotechnology, Bioprocess, Bioseparation, and Cell Technology as well as new articles (80 in all,) and features the same breadth and quality of coverage and clarity of presentation found in the original. For instructors, advanced students, and those involved in regulatory compliance, this two-volume desk reference offers an accessible and comprehensive resource. Zusammenfassung Biotechnology represents a major area of research focus, and many universities are developing academic programs in the field. Inhaltsverzeichnis VOLUME I: EXPRESSION SYSTEMS & PROCESS DEVELOPMENT PREFACE xi CONTRIBUTORS xiii PART I INTRODUCTION 3 PART II INDUSTRIAL CELL GROWTH AND GENE EXPRESSION SYSTEMS 7 1 Animal Cells, Suspension Culture 9 John R. Birch 2 Baculovirus Expression Systems 19 Robert D. Possee, Richard B. Hitchman, and Linda A. King 3 Baculovirus Kinetics, Insect Culture 33 Leslie Chan, Steve Reid, and Lars Keld Nielsen 4 Cell Culture, Aseptic Techniques 49 John M. Davis and Kevin L. Shade 5 Cell Cycle in Bioprocesses 71 Mariam Naciri and Mohamed Al-rubeai 6 Cell Growth and Protein Expression Kinetics 85 Dhinakar S. Kompala 7 Cell Viability Measurement 97 Ning Wei and Benjamin Sommer 8 Contamination Detection in Animal Cell Culture 105 Carol Mclean and Colin Harbour 9 Culture Collections and Biological Resource Centers (BRCs) 131 David Smith 10 Culture Preservation 153 Robert L. Gherna 11 Expression and Secretion of Heterologous Proteins, Bacillus and Other Gram-Positive Bacteria 163 Boyke Bunk, Rebekka Biedendieck, Dieter Jahn, and Patricia S. Vary 12 Gene Expression in Human Cells 181 Marco A. Cacciuttolo, Gene Lee, John Chon, and John Lewis 13 Gene expression in Pichia and other methylotroph yeast 195 Koti Sreekrishna 14 Gene Expression in Recombinant Animal Cells and Transgenic Animals 213 Richard M. Twyman and Bruce Whitelaw 15 Inoculum Expansion Methods, Animal Cell Lines 297 Claudia Kloth, Glenn Maclsaac, Haile Ghebremariam, and Alahari Arunakumari 16 Insect Cell Culture 311 Someet Narang, Erik M. Whiteley, Sunyia Hussain, and Michael J. Betenbaugh 17 K...
