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It is refreshing to find a book treating alkaloid analysis with all the latest techniques such as inverse-detected two dimensional NMR, GC/MS, and HPLC associated techniques. There have been many developments in the field of alkaloid analysis since the 1950s, not the least of which are the various powerful spectroscopic methods, which dominate this volume. Included also are the various aspects of tobacco alkaloids and analysis in various situations. The complexities of sampling and analysis in tobacco smoke is explained. Analysis of alkaloids produced by tobacco root cell cultures is included, while genetic and chemical analysis is described for Papaver, a plant of considerable medicinal significance.
Inhaltsverzeichnis
Isolation, Identification, and Structure Elucidation of Alkaloids A General Overview.- 1 Introduction.- 2 Isolation.- 3 Identification and Structure Elucidation.- 4 Strategy.- 5 Future Prospects.- References.- Inverse-Detected 2D-NMR Applications in Alkaloid Chemistry.- 1 Introduction.- 2 Historical Background.- 3 Suppression of Unwanted Magnetization Arising from 1H-12C Species.- 4 Inverse-Detected One-Bond Heteronuclear Shift Correlation Experiments.- 5 Long-Range Heteronuclear Chemical Shift Correlation - HMBC.- 6 HMQC-TOCSY and Other Hyphenated Inverse-Detected 2D Experiments.- 7 Other Inverse-Detected NMR Experiments -Selective and F1 Region-Selected Experiments.- 8 Sample Requirements.- 9 Applications of Inverse-Detected NMR Techniques to Alkaloid Chemistry. Structural Problems.- 10 Conclusion.- References.- Electrochemical Detection of Alkaloids in HPLC.- 1 Introduction.- 2 Principles of HPLC with Electrochemical Detection.- 3 Methods of Detection in Plants.- 4 Possible Methods for Detecting Other Alkaloids.- 5 Conclusions and Future Directions.- References.- Gas Chromatography in the Analysis of Alkaloids.- 1 Introduction.- 2 Pyrrolizidine Alkaloids.- 3 Quinolizidine Alkaloids.- 4 Tropane Alkaloids.- 5 Quinoline Alkaloids.- 6 Morphinan Alkaloids.- 7 Terpenoid Indole Alkaloids.- 8 Ergot Indole Alkaloids.- 9 Steroidal Alkaloids.- 10 Diterpenoid Alkaloids.- 11 Other Alkaloids.- 12 Conclusion.- References.- Alkaloid Analysis in Flue-Cured Tobacco.- 1 Introduction.- 2 Factors Affecting Alkaloid Concentration.- 3 Methods of Analysis of Tobacco Alkaloids.- 4 Summary.- References.- Assessment of Burley and Dark Tobacco Alkaloids During Storage, Aging, and Fermentation.- 1 Importance of Pyridine Alkaloids to Tobacco Quality and Usability.- 2 Importance of Storage, Aging,and Fermentation.- 3 Chemical Analyses.- References.- Detection of Alkaloids in Environmental Tobacco Smoke.- 1 Introduction.- 2 Overview of Sampling and Analysis Methods.- 3 Time-Integrated Sampling/Gas Chromatographic Analysis.- 4 Real-Time Sampling/Mass Spectrometric Analysis.- 5 Summary.- References.- Methods for Production of Alkaloids in Root Cultures and Analysis of Products.- 1 Introduction.- 2 Induction of Transformed Roots.- 3 Proof of Transformation.- 4 Alkaloid Extraction and Quantification.- References.- Genetic and Chemical Analysis for Alkaloids in Papaver.- 1 Introduction.- 2 Genetics of Major Opium Alkaloids and Importance of Chemical Analysis.- 3 Chemical Analysis of Major Opium Alkaloids.- 4 Concluding Remarks.- References.
