DNA Repair Protocols

In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair. This collection of readily reproducible techniques for repairing mammalian DNA contains fourteen entirely new chapters and many extensively revised chapters. The methods presented cover cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting.

Inhaltsverzeichnis

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
Malgorzata Z. Zdzienicka
Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes
Mathilde Fréchet, Valérie Bergoglio, Odile Chevallier-Lagente, Alain Sarasin, and Thierry Magnaldo
Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities
William W. Au and Salama A. Salama
Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation
Shruti Nagar, James J. Corcoran, and William F. Morgan
Inhibition of DNA Synthesis by Ionizing Radiation: A Marker for an S-Phase Checkpoint
Nicolaas G. J. Jaspers and Malgorzata Z. Zdzienicka
Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
George Iliakis, Ya Wang, and Hong Yan Wang
Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage
Xuan Huang and Zbigniew Darzynkiewicz
Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication)
Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi
In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
George Iliakis and Nge Cheong
Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
Michael J. Villalobos, Christopher J. Betti, and Andrew T. M Vaughan
Plasmid-Based Assays for DNA End-Joining In Vitro
George Iliakis, Bustanur Rosidi, Minli Wang, and Huichen Wang
Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants
Rodney S. Nairn and Gerald M. Adair
Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr
Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells
Janine H. Santos, Joel N. Meyer, Bhaskar S. Mandavilli, and Bennett Van Houten
Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR
Gerd P. Pfeifer
Immunochemical Detection of UV-Induced DNA Damage and Repair
Marcus S. Cooke and Alistair Robson
A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts
Shirley McCready
Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
David L. Mitchell
DNA Damage Quantitation by Alkaline Gel Electrophoresis
Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland
The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Günter Speit and Andreas Hartmann
Fast Micromethod DNA Single-Strand-Break Assay
Heinz C. Schröder, Renato Batel, Heiko Schwertner, Oleksandra Boreiko, and Werner E. G. Müller
32P-Postlabeling DNA Damage Assays: PAGE, TLC, and HPLC
Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki
Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA
Vaughn Smider, Byung Joon Hwang, and Gilbert Chu
Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision
Huixian Wang and John B. Hays
Characterization of Enzymes that Initiate Base Excision Repair at Abasic Sites
Walter A. Deutsch and Vijay Hegde
Base Excision Repair in Mammalian Cells
Yoshihiro Matsumoto
In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
Guido Frosina, Enrico Cappelli, Monica Ropolo, Paola Fortini, Barbara Pascucci, and Eugenia Dogliotti
Biochemical Assays for the Characterization of DNA Helicases
Robert M. Brosh, Jr. and Sudha Sharma
Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA
Maureen Biggerstaff and Richard D. Wood
Assaying for the Dual Incisions of Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site
Mahmud K. K. Shivji, Jonathan G. Moggs, Isao Kuraoka, and Richard D. Wood
Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated with DNA Excision Repair Sites in Mammalian Cells
A. Ivana Scovassi

Zusammenfassung

The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.

Zusatztext

"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research

"...must for any science library supporting a molecular biology program." - E-Streams

"An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie
"...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair.  Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts." -Int. J. Radiation Oncology Biol. Phys.
"This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety." -BTS Newsletter

Bericht

"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research"...must for any science library supporting a molecular biology program." - E-Streams"An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie "...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair. Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts." -Int. J. Radiation Oncology Biol. Phys. "This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety." -BTS Newsletter

Produktdetails

• Autoren: Daryl S. Henderson
• Mitarbeit: Daryl S. Henderson (Herausgeber), Dary S Henderson (Herausgeber), Daryl S Henderson (Herausgeber)
• Verlag: Springer, Berlin
• Sprache: Englisch
• Produktform: Taschenbuch
• Erschienen: 26.10.2010
• EAN: 9781617376115
• ISBN: 978-1-61737-611-5
• Seiten: 512
• Illustration: 105 SW-Abb.
• Serien: Methods in Molecular Biology; Methods in Molecular Biology
• Themen: Naturwissenschaften, Medizin, Informatik, Technik > Biologie > Biochemie, Biophysik; B, Zellbiologie (Zytologie), Life Sciences, biochemistry, Biomedical and Life Sciences, Cellular biology (cytology), Biochemistry, general, Cell Biology, genes, recombination, reproducible techniques